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Structure of activated SAVED-CHAT filament
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8tl0
Q-score: 0.485
Steens JA, Bravo JPK, Salazar CRP, Yildiz C, Amieiro AM, Kostlbacher S, Prinsen SHP, Andres AS, Patinios C, Bardis A, Barendregt A, Scheltema RA, Ettema TJG, van der Oost J, Taylor DW, Staals RHJ
Science (2024) 383 pp. 512-519 [ Pubmed: 38301007 DOI: doi:10.1126/science.adk0378 ]
- Chat domain-containing protein (54 kDa, Protein from Haliangium ochraceum)
- Rna (5'-r(*ap*ap*a)-3') (942 Da, RNA from synthetic construct)
- Active saved-chat filament (1 MDa, Complex from Haliangium ochraceum)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7jhy
Q-score: 0.323
Zhou Y, Bravo JPK, Taylor HN, Steens JA, Jackson RN, Staals RHJ, Taylor DW
iScience (2021) 24 pp. 102201-102201 [ Pubmed: 33733066 DOI: doi:10.1016/j.isci.2021.102201 ]
- Csf2 (cas7) (32 kDa, Protein from Mycobacterium sp. JS623)
- Rna (31-mer) (9 kDa, RNA from Mycobacterium sp. JS623)
- Csf4 (cas11) (17 kDa, Protein from Mycobacterium sp. JS623)
- Csf crispr complex (Complex from Mycobacterium sp. JS623)
Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA
Science (2015) 348 pp. 581-585 [ Pubmed: 25837515 DOI: doi:10.1126/science.aaa4535 ]
- Apo crispr-cmr complex (365 kDa, Sample)
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Crispr rna (RNA from Thermus thermophilus)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA
Science (2015) 348 pp. 581-585 [ Pubmed: 25837515 DOI: doi:10.1126/science.aaa4535 ]
- Smaller apo crispr-cmr complex (316 kDa, Sample)
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Crispr rna (RNA from Thermus thermophilus)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA
Science (2015) 348 pp. 581-585 [ Pubmed: 25837515 DOI: doi:10.1126/science.aaa4535 ]
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Target rna (16 kDa, RNA from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Crispr rna (RNA from Thermus thermophilus)
- Target-bound crispr-cmr complex (381 kDa, Sample)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
RNA-targeting by the Type III-A CRISPR-Cas Csm complex of Thermus thermophilus
Staals RHJ, Zhu Y, Taylor DW, Kornfeld JE, Sharma K, Barendregt A, Koehorst JJ, Vlot M, Neupane N, Varossieau K, Sakamoto K, Suzuki T, Dohmae N, Yokoyama S, Schaap PJ, Urlaub H, Heck AJR, Nogales E, Doudna JA, Shinkai A, van der Oost J
Mol Cell (2014) 56 pp. 518-530 [ DOI: doi:10.1016/j.molcel.2014.10.005 Pubmed: 25457165 ]
- Csm4 (32 kDa, Protein from Thermus thermophilus)
- Crispr rna (14 kDa, RNA from Thermus thermophilus)
- Csm2 (16 kDa, Protein from Thermus thermophilus)
- Csm5 (45 kDa, Protein from Thermus thermophilus)
- Csm3 (27 kDa, Protein from Thermus thermophilus)
- Csm1 (90 kDa, Protein from Thermus thermophilus)
- Type iii-a crispr-cas csm complex of thermus thermophilus (426 kDa, Sample)
Structure and activity of an RNA-targeting Type III-B CRISPER-Cas complex
Staals RH, Agari Y, Maki-Yonekura S, Zhu Y, Taylor DW, van Duijn E, Barendregt A, Vlot M, Koehorst JJ, Sakamoto K, Masuda A, Dohmae N, Schaap PJ, Doudna JA, Heck AJ, Yonekura K, van der Oost J, Shinkai A
Mol Cell (2013) 52 pp. 135-145 [ DOI: doi:10.1016/j.molcel.2013.09.013 Pubmed: 24119403 ]
- Type iii-b crispr-cas complex of thermus thermophilus (365 kDa, Sample)
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Crispr rna (RNA from Thermus thermophilus)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)