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Structure of 2x duplex target DNA-bound Cascade/I-C
Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
Mol Cell (2016) 63 pp. 840-851 [ Pubmed: 27588603 DOI: doi:10.1016/j.molcel.2016.07.027 ]
- Apo-cascade/i-c (342 kDa, Complex from Desulfovibrio vulgaris)
Structure of 2x duplex DNA target-bound Cascade/I-C
Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
Mol Cell (2016) 63 pp. 840-851 [ Pubmed: 27588603 DOI: doi:10.1016/j.molcel.2016.07.027 ]
Structure of 1x duplex DNA target-bound Cascade/I-C
Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
Mol Cell (2016) 63 pp. 840-851 [ DOI: doi:10.1016/j.molcel.2016.07.027 Pubmed: 27588603 ]
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Cas9 bound to single guide-rna (194 kDa, Sample)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Target 40-bp double stranded dna (24 kDa, DNA)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Cas9 bound to single guide-rna and 40-bp target dna (218 kDa, Sample)
Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA
Science (2015) 348 pp. 581-585 [ Pubmed: 25837515 DOI: doi:10.1126/science.aaa4535 ]
- Apo crispr-cmr complex (365 kDa, Sample)
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Crispr rna (RNA from Thermus thermophilus)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA
Science (2015) 348 pp. 581-585 [ Pubmed: 25837515 DOI: doi:10.1126/science.aaa4535 ]
- Smaller apo crispr-cmr complex (316 kDa, Sample)
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Crispr rna (RNA from Thermus thermophilus)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA
Science (2015) 348 pp. 581-585 [ Pubmed: 25837515 DOI: doi:10.1126/science.aaa4535 ]
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Target rna (16 kDa, RNA from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Crispr rna (RNA from Thermus thermophilus)
- Target-bound crispr-cmr complex (381 kDa, Sample)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
RNA-targeting by the Type III-A CRISPR-Cas Csm complex of Thermus thermophilus
Staals RHJ, Zhu Y, Taylor DW, Kornfeld JE, Sharma K, Barendregt A, Koehorst JJ, Vlot M, Neupane N, Varossieau K, Sakamoto K, Suzuki T, Dohmae N, Yokoyama S, Schaap PJ, Urlaub H, Heck AJR, Nogales E, Doudna JA, Shinkai A, van der Oost J
Mol Cell (2014) 56 pp. 518-530 [ DOI: doi:10.1016/j.molcel.2014.10.005 Pubmed: 25457165 ]
- Csm4 (32 kDa, Protein from Thermus thermophilus)
- Crispr rna (14 kDa, RNA from Thermus thermophilus)
- Csm2 (16 kDa, Protein from Thermus thermophilus)
- Csm5 (45 kDa, Protein from Thermus thermophilus)
- Csm3 (27 kDa, Protein from Thermus thermophilus)
- Csm1 (90 kDa, Protein from Thermus thermophilus)
- Type iii-a crispr-cas csm complex of thermus thermophilus (426 kDa, Sample)
CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference
Hochstrasser ML, Taylor DW, Bhat P, Guegler CK, Sternberg SH, Nogales E, Doudna JA
PNAS (2014) 111 pp. 6618-6623 [ Pubmed: 24748111 DOI: doi:10.1073/pnas.1405079111 ]
- Crispr system cascade subunit casa (55 kDa, Protein from Escherichia coli K-12)
- Crispr system cascade subunit case (22 kDa, Protein from Escherichia coli K-12)
- Crispr system cascade subunit casb (18 kDa, Protein from Escherichia coli K-12)
- 72 bp dsdna target with r44 protospacer (44 kDa, DNA from Enterobacteria phage P7)
- R44 crispr rna (18 kDa, RNA from Escherichia coli K-12)
- Cascade bound to a 72 bp target dsdna (443 kDa, Sample)
- Crispr system cascade subunit casd (25 kDa, Protein from Escherichia coli K-12)
- Crispr system cascade subunit casc (40 kDa, Protein from Escherichia coli K-12)