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Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 5lcw
Q-score: 0.252
Alfieri C, Chang L, Zhang Z, Yang J, Maslen S, Skehel M, Barford D
Nature (2016) 536 pp. 431-436 [ Pubmed: 27509861 DOI: doi:10.1038/nature19083 ]
- Cell division cycle protein 20 homolog (41 kDa, Protein from Homo sapiens)
- Mitotic checkpoint serine/threonine-protein kinase bub1 beta,mitotic checkpoint serine/threonine-protein kinase bub1 beta (39 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 11 (9 kDa, Protein from Homo sapiens)
- Cell division cycle protein 27 homolog (91 kDa, Protein from Homo sapiens)
- Mitotic spindle assembly checkpoint protein mad2a (23 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 2 (93 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 4 (92 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 5 (85 kDa, Protein from Homo sapiens)
- Cell division cycle protein 23 homolog (68 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit cdc26 (9 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 1 (216 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 10 (21 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 7 (66 kDa, Protein from Homo sapiens)
- Cell division cycle protein 20 homolog (54 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 13 (8 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 16 (11 kDa, Protein from Homo sapiens)
- Cell division cycle protein 16 homolog (71 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 15 (14 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex/cyclosome, in complex with the mitotic checkpoint complex in closed conformation (apc/c-mcc-closed) at 4.2 angstrom resolution (1 MDa, Complex from Homo sapiens)
Cryo-EM structure of human apo unphosphorylated APC/C
Zhang S, Chang L, Alfieri C, Zhang Z, Yang J, Maslen S, Skehel M, Barford D
Nature (2016) 533 pp. 260-264 [ DOI: doi:10.1038/nature17973 Pubmed: 27120157 ]
- Anaphase-promoting complex subunit 11 (9 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 8 (68 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 16 (11 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 6 (71 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 2 (93 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 7 (66 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 13 (8 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 4 (92 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 3 (91 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 5 (85 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 15 (14 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 1 (216 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 10 (21 kDa, Protein from Homo sapiens)
- Recombinant human apo unphosphorylated apc/c (1 MDa, Sample)
- Anaphase-promoting complex subunit 12 (9 kDa, Protein from Homo sapiens)
Cryo-EM structure of combined apo phosphorylated APC/C
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5g05
Q-score: 0.375
Zhang S, Chang L, Alfieri C, Zhang Z, Yang J, Maslen S, Skehel M, Barford D
Nature (2016) 533 pp. 260-264 [ DOI: doi:10.1038/nature17973 Pubmed: 27120157 ]
- Anaphase-promoting complex subunit 11 (9 kDa, Protein from Homo sapiens)
- Recombinant human apo apc/c phosphorylated in vitro (1 MDa, Sample)
- Anaphase-promoting complex subunit 8 (68 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 16 (11 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 6 (71 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 2 (93 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 7 (66 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 13 (8 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 4 (92 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 5 (85 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 15 (14 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 1 (216 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 10 (21 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 3 (91 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 12 (9 kDa, Protein from Homo sapiens)
Cryo-EM structure of a human APC/C mutant with Apc1 300s loop deleted
Zhang S, Chang L, Alfieri C, Zhang Z, Yang J, Maslen S, Skehel M, Barford D
Nature (2016) 533 pp. 260-264 [ DOI: doi:10.1038/nature17973 Pubmed: 27120157 ]
- Anaphase-promoting complex subunit 11 (9 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 8 (68 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 16 (11 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 6 (71 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 2 (93 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 7 (66 kDa, Protein from Homo sapiens)
- Human apc/c mutant with apc1 300s loop deleted (1 MDa, Sample)
- Anaphase-promoting complex subunit 13 (8 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 4 (92 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 5 (85 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 15 (14 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 1 (216 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 10 (21 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 3 (91 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 12 (9 kDa, Protein from Homo sapiens)
Cryo-EM structure of a human APC/C mutant with Apc1 300s loop deleted in complex with TAME
Zhang S, Chang L, Alfieri C, Zhang Z, Yang J, Maslen S, Skehel M, Barford D
Nature (2016) 533 pp. 260-264 [ DOI: doi:10.1038/nature17973 Pubmed: 27120157 ]
- Anaphase-promoting complex subunit 11 (9 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 8 (68 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 16 (11 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 6 (71 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 2 (93 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 7 (66 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 13 (8 kDa, Protein from Homo sapiens)
- Recombinant human apc/c mutant with apc1 300s loop deleted in complex with tame (1 MDa, Sample)
- Anaphase-promoting complex subunit 4 (92 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 5 (85 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 15 (14 kDa, Protein from Homo sapiens)
- Tosyl-l-arginine methyl ester (Ligand from unidentified)
- Anaphase-promoting complex subunit 1 (216 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 10 (21 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 3 (91 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 12 (9 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human APC/C-Cdc20-Hsl1 complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5g04
Q-score: 0.299
Zhang S, Chang L, Alfieri C, Zhang Z, Yang J, Maslen S, Skehel M, Barford D
Nature (2016) 533 pp. 260-264 [ DOI: doi:10.1038/nature17973 Pubmed: 27120157 ]
- Anaphase-promoting complex subunit 11 (9 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 8 (68 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 16 (11 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 6 (71 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 2 (93 kDa, Protein from Homo sapiens)
- Hsl1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Anaphase-promoting complex subunit 7 (66 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 13 (8 kDa, Protein from Homo sapiens)
- Cell division cycle protein 20 homolog (54 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 4 (92 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 5 (85 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 15 (14 kDa, Protein from Homo sapiens)
- Recombinant human apc/c-cdc20-hsl1 complex (1 MDa, Sample)
- Anaphase-promoting complex subunit 1 (216 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 10 (21 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 3 (91 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 12 (9 kDa, Protein from Homo sapiens)
Cryo-EM structure of human apo phosphorylated APC/C
Zhang S, Chang L, Alfieri C, Zhang Z, Yang J, Maslen S, Skehel M, Barford D
Nature (2016) 533 pp. 260-264 [ DOI: doi:10.1038/nature17973 Pubmed: 27120157 ]
- Anaphase-promoting complex subunit 11 (9 kDa, Protein from Homo sapiens)
- Recombinant human apo apc/c phosphorylated in vitro (1 MDa, Sample)
- Anaphase-promoting complex subunit 8 (68 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 16 (11 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 6 (71 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 2 (93 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 7 (66 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 13 (8 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 4 (92 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 5 (85 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 15 (14 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 1 (216 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 10 (21 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 3 (91 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 12 (9 kDa, Protein from Homo sapiens)