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EMD-10503

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Cryo-EM Structure of T. kodakarensis 70S ribosome

Release date: July 29, 2020
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 6th6
Q-score: 0.622

Sas-Chen A, Thomas JM, Matzov D, Taoka M, Nance KD, Nir R, Bryson KM, Shachar R, Liman GLS, Burkhart BW, Gamage ST, Nobe Y, Briney CA, Levy MJ, Fuchs RT, Robb GB, Hartmann J, Sharma S, Lin Q, Florens L, Washburn MP, Isobe T, Santangelo TJ, Shalev-Benami M, Meier JL, Schwartz S

Nature (2020) 583 pp. 638-643 [ DOI: doi:10.1038/s41586-020-2418-2 Pubmed: 32555463 ]

Sample:

30s ribosomal protein s9 (15 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l2 (26 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s4 (21 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l18e (13 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l29 (7 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l24 (14 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s3 (23 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l14 (15 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
5s ribosomal rna (40 kDa, RNA from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l23 (9 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l34e (10 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l40e (5 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s17 (13 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s14 type z (6 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
16s ribosomal rna (486 kDa, RNA from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
70s ribosome from thermococcus kodakarensis (Complex from Thermococcus kodakarensis)
30s ribosomal protein s6e (13 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s27e (7 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l37ae (9 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l15e (22 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l18 (22 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
Water (18 Da, Ligand)
30s ribosomal protein s19 (15 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l6 (20 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l22 (17 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l37e (7 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l39e (6 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
23s ribosomal rna (987 kDa, RNA from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l21e (11 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l19e (17 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l10e (21 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s3ae (23 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s24e (11 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s28e (7 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l13 (16 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s5 (26 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s4e (27 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s17e (8 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l5 (21 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l30 (17 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
Lsu ribosomal protein l41e (5 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s7 (24 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l24e (8 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s11 (15 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s13 (17 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l18ae (9 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s8 (14 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s15 (17 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l15 (16 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l32e (14 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
Zinc ion (65 Da, Ligand)
30s ribosomal protein s19e (17 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l31e (10 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l4 (28 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s10 (11 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s8e (14 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s12 (16 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l14e (8 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l7ae (13 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l44e (11 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l3 (39 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
30s ribosomal protein s2 (23 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
Predicted zinc-ribbon rna-binding protein involved in translation (7 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))
50s ribosomal protein l30e (10 kDa, Protein from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1))

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