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Cryo-EM structure of the light-driven sodium pump ErNaR in the pentameric form at pH 8.0
Resolution: 2.63 Å
EM Method: Single-particle
Fitted PDBs: 8qqz
Q-score: 0.68
Podoliak E, Lamm GHU, Marin E, Schellbach AV, Fedotov DA, Stetsenko A, Asido M, Maliar N, Bourenkov G, Balandin T, Baeken C, Astashkin R, Schneider TR, Bateman A, Wachtveitl J, Schapiro I, Busskamp V, Guskov A, Gordeliy V, Alekseev A, Kovalev K
Nat Commun (2024) 15 pp. 3119-3119 [ DOI: doi:10.1038/s41467-024-47469-0 Pubmed: 38600129 ]
- Eicosane (282 Da, Ligand)
- Bacteriorhodopsin-like protein (32 kDa, Protein from Erythrobacter)
- Light-driven sodium pump ernar (36 kDa, Complex from Erythrobacter)
- Water (18 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
Cryo-EM structure of the light-driven sodium pump ErNaR in the pentameric form at pH 4.3
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 8qr0
Q-score: 0.687
Podoliak E, Lamm GHU, Marin E, Schellbach AV, Fedotov DA, Stetsenko A, Asido M, Maliar N, Bourenkov G, Balandin T, Baeken C, Astashkin R, Schneider TR, Bateman A, Wachtveitl J, Schapiro I, Busskamp V, Guskov A, Gordeliy V, Alekseev A, Kovalev K
Nat Commun (2024) 15 pp. 3119-3119 [ DOI: doi:10.1038/s41467-024-47469-0 Pubmed: 38600129 ]
- Bacteriorhodopsin-like protein (32 kDa, Protein from Erythrobacter)
- Eicosane (282 Da, Ligand)
- Light-driven sodium pump ernar (36 kDa, Complex from Erythrobacter)
- Water (18 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
Cryo-EM structure of a Staphylococus aureus 30S-RbfA complex
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8byv
Q-score: 0.383
Bikmullin AG, Fatkhullin B, Nurullina L, Garaeva N, Klochkova E, Guskov A, Stetsenko A, Khusainov I, Golubev A, Gabdulkhakov A, Trachtman N, Blokhin D, Validov S, Usachev K, Yusupov M
To Be Published
- 30s small ribosomal subunit (Complex from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s13 (13 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s small ribosomal subunit with ribosome binding factor a (Complex)
- 30s ribosomal protein s11 (13 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s19 (10 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- Ribosome-binding factor a (13 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s8 (14 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s2 (29 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s6 (11 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s3 (24 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s20 (9 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s15 (10 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s10 (11 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s4 (23 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s7 (17 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s17 (10 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s12 (15 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 16s ribosomal rna (500 kDa, RNA from Staphylococcus aureus subsp. aureus NCTC 8325)
- Ribosome-binding factor a (Complex from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s9 (14 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s5 (17 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s16 (10 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s18 (9 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
Cryo-EM map of the Candida albicans 80S ribosome in complex with cephaeline
Kolosova O, Zgadzay Y, Stetsenko A, Atamas A, Wu C, Jenner L, Sachs SM, Guskov A, Yusupov M
Biopolym Cell (2023) [ DOI: doi:10.7124/bc.000AA4 ]
The cryo-EM map of the C. albicans ribosome focused on the head of the SSU
Kolosova O, Zgadzay Y, Stetsenko A, Atamas A, Wu C, Jenner L, Sachs SM, Guskov A, Yusupov M
Biopolym Cell (2023) [ DOI: doi:10.7124/bc.000AA4 ]
Combined map of Candida albicans 80S ribosome in complex with cephaeline
Kolosova O, Zgadzay Y, Stetsenko A, Atamas A, Wu C, Jenner L, Sachs SM, Guskov A, Yusupov M
Biopolym Cell (2023) [ DOI: doi:10.7124/bc.000AA4 ]
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 8afa
Q-score: 0.516
Colucci E, Anshari ZR, Patino-Ruiz MF, Nemchinova M, Whittaker J, Slotboom DJ, Guskov A
Nat Commun (2023) 14 pp. 1799-1799 [ Pubmed: 37002226 DOI: doi:10.1038/s41467-023-37503-y ]
Structure of Candida albicans 80S ribosome in complex with cycloheximide
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 7q08
Q-score: 0.517
Zgadzay Y, Kolosova O, Stetsenko A, Wu C, Bruchlen D, Usachev K, Validov S, Jenner L, Rogachev A, Yusupova G, Sachs MS, Guskov A, Yusupov M
Sci Adv (2022) 8 pp. eabn1062-eabn1062 [ DOI: doi:10.1126/sciadv.abn1062 Pubmed: 35613268 ]
- Ribosomal 60s subunit protein l3 (44 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l36 (11 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s20 (13 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s15 (15 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l10 (25 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l34b (13 kDa, Protein from Candida albicans SC5314)
- Ribosomal protein l37 (10 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s13 (16 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein el41 (3 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s23b (16 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s8 (22 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s1 (29 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l42a (12 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l7a (27 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s21 (9 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l31b (12 kDa, Protein from Candida albicans SC5314)
- Ribosomal protein l15 (24 kDa, Protein from Candida albicans SC5314)
- 5s ribosomal rna (38 kDa, RNA from Candida albicans SC5314)
- Ribosomal 40s subunit protein s18b (17 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s17b (15 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s4 (29 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s2 (26 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s26 (13 kDa, Protein from Candida albicans SC5314)
- Spermidine (145 Da, Ligand)
- Ribosomal 60s subunit protein l5 (34 kDa, Protein from Candida albicans SC5314)
- Ubiquitin-ribosomal 40s subunit protein s31 fusion protein (22 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s6 (27 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l22b (14 kDa, Protein from Candida albicans SC5314)
- 5.8s ribosomal rna (50 kDa, RNA from Candida albicans SC5314)
- Ribosomal 40s subunit protein s19a (16 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s30 (7 kDa, Protein from Candida albicans SC5314)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s16a (15 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l28 (16 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l17b (21 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l33a (12 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s11a (17 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s3 (27 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l25 (15 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s14b (14 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s22-a (14 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l39 (6 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l35a (14 kDa, Protein from Candida albicans SC5314)
- 18s ribosomal rna (575 kDa, RNA from Candida albicans SC5314)
- Ribosomal protein l19 (21 kDa, Protein from Candida albicans SC5314)
- Habp4_pai-rbp1 domain-containing protein (29 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l26b (14 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l4b (39 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l27 (15 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l16a (22 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s24 (15 kDa, Protein from Candida albicans SC5314)
- 25s ribosomal rna (1 MDa, RNA from Candida albicans SC5314)
- Ribosomal 40s subunit protein s28b (7 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l29 (7 kDa, Protein from Candida albicans SC5314)
- Zinc ion (65 Da, Ligand)
- Ribosomal 60s subunit protein l24a (17 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l14b (14 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l43a (10 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l6 (19 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l18a (20 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l2a (27 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s12 (15 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l8 (28 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s9b (21 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s29a (6 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l11b (19 kDa, Protein from Candida albicans SC5314)
- 4-{(2r)-2-[(1s,3s,5s)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione (281 Da, Ligand)
- 40s ribosomal protein s25 (11 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l21a (18 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s5 (25 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s10a (13 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s0 (28 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l38 (8 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l23b (14 kDa, Protein from Candida albicans SC5314)
- Candida albicans 80s ribosome (3 MDa, Complex from Candida albicans SC5314)
- Ribosomal 60s subunit protein l32 (14 kDa, Protein from Candida albicans SC5314)
- Rpl40bp (6 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l9b (21 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l20 (20 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s27 (8 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s7 (21 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l13 (23 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l30 (11 kDa, Protein from Candida albicans SC5314)
Cryo-EM map of the Candida albicans 80S ribosome in complex with blasticidin s
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 7q0r
Q-score: 0.448
Zgadzay Y, Kolosova O, Stetsenko A, Wu C, Bruchlen D, Usachev K, Validov S, Jenner L, Rogachev A, Yusupova G, Sachs MS, Guskov A, Yusupov M
Sci Adv (2022) 8 pp. eabn1062-eabn1062 [ DOI: doi:10.1126/sciadv.abn1062 Pubmed: 35613268 ]
- 60s ribosomal protein l36 (11 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s20 (13 kDa, Protein from Candida albicans SC5314)
- Blasticidin s (422 Da, Ligand)
- Ribosomal 40s subunit protein s15 (15 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l10 (25 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l34b (13 kDa, Protein from Candida albicans SC5314)
- Ribosomal protein l37 (10 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s13 (16 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l41 (3 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s23b (16 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s8 (22 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s1 (29 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l42a (12 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l7a (27 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s21 (9 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l31b (12 kDa, Protein from Candida albicans SC5314)
- Ribosomal protein l15 (24 kDa, Protein from Candida albicans SC5314)
- 5s ribosomal rna (38 kDa, RNA from Candida albicans SC5314)
- Ribosomal 40s subunit protein s18b (17 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s17b (15 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s4 (29 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s2 (26 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s26 (13 kDa, Protein from Candida albicans SC5314)
- Spermidine (145 Da, Ligand)
- Ribosomal 60s subunit protein l5 (34 kDa, Protein from Candida albicans SC5314)
- Ubiquitin-ribosomal 40s subunit protein s31 fusion protein (22 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s6 (27 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l22b (14 kDa, Protein from Candida albicans SC5314)
- 5.8s ribosomal rna (50 kDa, RNA from Candida albicans SC5314)
- Ribosomal 40s subunit protein s19a (16 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s30 (7 kDa, Protein from Candida albicans SC5314)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s16a (15 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l28 (16 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l17b (21 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l33a (12 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s11a (17 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s3 (27 kDa, Protein from Candida albicans SC5314)
- Mixture of the endogenous e-trna (24 kDa, RNA from Candida albicans SC5314)
- Ribosomal 60s subunit protein l25 (15 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s14b (14 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s22-a (14 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l39 (6 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l35a (14 kDa, Protein from Candida albicans SC5314)
- 18s ribosomal rna (575 kDa, RNA from Candida albicans SC5314)
- Ribosomal protein l19 (21 kDa, Protein from Candida albicans SC5314)
- Habp4_pai-rbp1 domain-containing protein (29 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l26b (14 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l4b (39 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l27 (15 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l16a (22 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s24 (15 kDa, Protein from Candida albicans SC5314)
- 25s ribosomal rna (1 MDa, RNA from Candida albicans SC5314)
- Ribosomal 40s subunit protein s28b (7 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l3 (44 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l29 (7 kDa, Protein from Candida albicans SC5314)
- Zinc ion (65 Da, Ligand)
- Ribosomal 60s subunit protein l24a (17 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l14b (14 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l43a (10 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l6 (19 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l18a (20 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l2a (27 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s12 (15 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l8 (28 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s9b (21 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s29a (6 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l11b (19 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s25 (11 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l21a (18 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s5 (25 kDa, Protein from Candida albicans SC5314)
- Ribosomal 40s subunit protein s10a (13 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s0 (28 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l38 (8 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l23b (14 kDa, Protein from Candida albicans SC5314)
- Candida albicans 80s ribosome (3 MDa, Complex from Candida albicans SC5314)
- Ribosomal 60s subunit protein l32 (14 kDa, Protein from Candida albicans SC5314)
- Rpl40bp (6 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l9b (21 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l20 (20 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s27 (8 kDa, Protein from Candida albicans SC5314)
- 40s ribosomal protein s7 (21 kDa, Protein from Candida albicans SC5314)
- 60s ribosomal protein l13 (23 kDa, Protein from Candida albicans SC5314)
- Ribosomal 60s subunit protein l30 (11 kDa, Protein from Candida albicans SC5314)