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Cryo-EM structure of DHEA-ADGRG2-BT-Gs complex
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7xkd
Q-score: 0.563
Lin H, Xiao P, Bu RQ, Guo S, Yang Z, Yuan D, Zhu ZL, Zhang CX, He QT, Zhang C, Ping YQ, Zhao RJ, Ma CS, Liu CH, Zhang XN, Jiang D, Huang S, Xi YT, Zhang DL, Xue CY, Yang BS, Li JY, Lin HC, Zeng XH, Zhao H, Xu WM, Yi F, Liu Z, Sun JP, Yu X
Nat Chem Biol (2022) 18 pp. 1196-1203 [ DOI: doi:10.1038/s41589-022-01084-6 Pubmed: 35982227 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nb35 (Complex from Mus musculus)
- Adhesion g-protein (Complex from Camelus bactrianus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2 (34 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2 (Complex)
- 3-beta-hydroxy-5-androsten-17-one (288 Da, Ligand)
- Nb35 (13 kDa, Protein from Camelus bactrianus)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2 (130 kDa, Complex from Homo sapiens)
Cryo-EM structure of DHEA-ADGRG2-FL-Gs complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7xke
Q-score: 0.532
Lin H, Xiao P, Bu RQ, Guo S, Yang Z, Yuan D, Zhu ZL, Zhang CX, He QT, Zhang C, Ping YQ, Zhao RJ, Ma CS, Liu CH, Zhang XN, Jiang D, Huang S, Xi YT, Zhang DL, Xue CY, Yang BS, Li JY, Lin HC, Zeng XH, Zhao H, Xu WM, Yi F, Liu Z, Sun JP, Yu X
Nat Chem Biol (2022) 18 pp. 1196-1203 [ DOI: doi:10.1038/s41589-022-01084-6 Pubmed: 35982227 ]
- Mini-gs (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2 (220 kDa, Complex from Mus musculus)
- Adhesion g-protein coupled receptor g2 (98 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- 3-beta-hydroxy-5-androsten-17-one (288 Da, Ligand)
- Nb35 (13 kDa, Protein from Camelus bactrianus)
- Water (18 Da, Ligand)
Cryo-EM structure of DHEA-ADGRG2-BT-Gs complex at lower state
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7xkf
Q-score: 0.563
Lin H, Xiao P, Bu RQ, Guo S, Yang Z, Yuan D, Zhu ZL, Zhang CX, He QT, Zhang C, Ping YQ, Zhao RJ, Ma CS, Liu CH, Zhang XN, Jiang D, Huang S, Xi YT, Zhang DL, Xue CY, Yang BS, Li JY, Lin HC, Zeng XH, Zhao H, Xu WM, Yi F, Liu Z, Sun JP, Yu X
Nat Chem Biol (2022) 18 pp. 1196-1203 [ DOI: doi:10.1038/s41589-022-01084-6 Pubmed: 35982227 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nb35 (Complex from Mus musculus)
- Adhesion g-protein (Complex from Camelus bactrianus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Receptor g2 (Complex)
- 3-beta-hydroxy-5-androsten-17-one (288 Da, Ligand)
- Adhesion g-protein coupled receptor g2 (34 kDa, Protein from Mus musculus)
- Nb35 (13 kDa, Protein from Camelus bactrianus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor g2 (130 kDa, Complex from Homo sapiens)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7wui
Q-score: 0.418
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ Pubmed: 35418677 DOI: doi:10.1038/s41586-022-04590-8 ]
- Adhesion g-protein coupled receptor g2,mcherry (126 kDa, Protein from Mus musculus)
- Mini-gs (41 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Cryo-em structure of the adgrg2-fl-ip15-gs complex (253 MDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Nanobody-35 (Complex from Lama glama)
- Adhesion g-protein coupled receptor g2 (Complex from Mus musculus)
- Ip15 (1 kDa, Protein from synthetic construct)
- Ip15 (Complex)
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Gs (Complex from Homo sapiens)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7wuj
Q-score: 0.474
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ Pubmed: 35418677 DOI: doi:10.1038/s41586-022-04590-8 ]
- Mini-gs (41 kDa, Protein from Homo sapiens)
- Cryo-em structure of the adgr4-beta-gs complex (Complex)
- Scfv16 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Nanobody-35 (Complex from Lama glama)
- Adhesion g-protein coupled receptor g4,uncharacterized protein (71 kDa, Protein from Psychromonas sp. B3M02)
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Gs (Complex from Homo sapiens)
Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7wuq
Q-score: 0.465
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature (2022) 604 pp. 771-778 [ Pubmed: 35418677 DOI: doi:10.1038/s41586-022-04590-8 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from Lama glama)
- Adhesion g-protein coupled receptor g2,mcherry (77 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from Lama glama)
- Adhesion g-protein coupled receptor g2 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gs (Complex from Homo sapiens)
- Adgrg2-beta-gs complex (134 kDa, Complex)
Cryo-EM structure of the P395-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfm
Q-score: 0.517
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ DOI: doi:10.1038/s41586-020-2569-1 Pubmed: 32698187 ]
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- P395 (Complex from synthetic construct)
- 2-(ethylamino)-6-[3-(4-propan-2-ylphenyl)propanoyl]-7,8-dihydro-5h-pyrido[4,3-d]pyrimidine-4-carboxamide (395 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- P395-bound gpbar-gs complex (Complex)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gpbar (Complex from Homo sapiens)
- Gs (Complex from Homo sapiens)
Cryo-EM structure of the INT-777-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfn
Q-score: 0.53
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ DOI: doi:10.1038/s41586-020-2569-1 Pubmed: 32698187 ]
- Nanobody-35 (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- (2s,4r)-4-[(3r,5s,6r,7r,8r,9s,10s,12s,13r,14s,17r)-6-ethyl-10,13-dimethyl-3,7,12-tris(oxidanyl)-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methyl-pentanoic acid (450 Da, Ligand)
- G-protein coupled bile acid receptor 1 (Complex)
- Int-777-bound gpbar-gs complex (Complex from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein subunits (Complex from synthetic construct)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)