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Cryo-EM structure of a chromatosome containing human linker histone H1.0
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7k5x
Q-score: 0.489
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 166-182.e6 [ DOI: doi:10.1016/j.molcel.2020.10.038 Pubmed: 33238161 ]
- Chromatosome containing human linker histone h1.0 (Complex from human)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Dna (197-mer) (61 kDa, DNA from Homo sapiens)
- Dna (197-mer) (60 kDa, DNA from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Scfv20 (29 kDa, Protein from Mus musculus)
- Histone h1.0 (20 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of a chromatosome containing human linker histone H1.4
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7k5y
Q-score: 0.527
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 166-182.e6 [ DOI: doi:10.1016/j.molcel.2020.10.038 Pubmed: 33238161 ]
- Chromatosome containing human linker histone h1.4 (Complex from human)
- Scfv20 (29 kDa, Protein from Mus musculus)
- Histone h1.4 (21 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Dna (197-mer) (61 kDa, DNA from Homo sapiens)
- Dna (197-mer) (60 kDa, DNA from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of a chromatosome containing human linker histone H1.10
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 7k60
Q-score: 0.435
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 166-182.e6 [ DOI: doi:10.1016/j.molcel.2020.10.038 Pubmed: 33238161 ]
- Scfv20 (29 kDa, Protein from Mus musculus)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h1.10 (22 kDa, Protein from Homo sapiens)
- Dna (197-mer) (61 kDa, DNA from Homo sapiens)
- Chromatosome containing human linker histone h1.10 (Complex from human)
- Dna (197-mer) (60 kDa, DNA from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of 197bp nucleosome aided by scFv
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 7k61
Q-score: 0.529
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 166-182.e6 [ DOI: doi:10.1016/j.molcel.2020.10.038 Pubmed: 33238161 ]
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Dna (197-mer) (61 kDa, DNA from Homo sapiens)
- Dna (197-mer) (60 kDa, DNA from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- 197bp nucleosome (Complex from human)
- Scfv20 (29 kDa, Protein from Mus musculus)
- Histone h4 (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of a chromatosome containing chimeric linker histone gH1.10-ncH1.4
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7k63
Q-score: 0.492
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 166-182.e6 [ DOI: doi:10.1016/j.molcel.2020.10.038 Pubmed: 33238161 ]
- Scfv20 (29 kDa, Protein from Mus musculus)
- Chromatosome containing chimeric linker histone gh1.10-nch1.4 (Complex from human)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Dna (197-mer) (61 kDa, DNA from Homo sapiens)
- Dna (197-mer) (60 kDa, DNA from Homo sapiens)
- Gh1.10-nch1.4 (23 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
cryo-EM map of di-chromatosome containing H1.4
Zhou BR, Feng H, Kale S, Fox T, Khant H, de Val N, Ghirlando R, Panchenko AR, Bai Y
Mol Cell (2021) 81 pp. 166-182.e6 [ DOI: doi:10.1016/j.molcel.2020.10.038 Pubmed: 33238161 ]
- Scfv20 (Protein from Mougeotia scalaris)
- Human histone h1.4 (Protein from Homo sapiens)
- Di-chromatosome containing human linker histone h1.4 (Complex from human)
- Human histone h3.1 (Protein from Homo sapiens)
- Human histone h4 (Protein from Homo sapiens)
- Human histone h2b1j (Protein from Homo sapiens)
- Dna(394-mer) (Protein from Homo sapiens)
- Human histone h2a1 (Protein from Homo sapiens)
- Dna (394-mer) (Protein from Homo sapiens)