Do data resources managed by EMBL-EBI and our collaborators make a difference to your work? Please take 10 minutes to fill in our annual user survey, and help us make the case for why sustaining open data resources is critical for life sciences research.
Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8fx5
Q-score: 0.595
Burger WAC, Pham V, Vuckovic Z, Powers AS, Mobbs JI, Laloudakis Y, Glukhova A, Wootten D, Tobin AB, Sexton PM, Paul SM, Felder CC, Danev R, Dror RO, Christopoulos A, Valant C, Thal DM
Nat Commun (2023) 14 pp. 5440-5440 [ DOI: doi:10.1038/s41467-023-41199-5 Pubmed: 37673901 ]
- Xanomeline (281 Da, Ligand)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Homo sapiens)
- M4 machr bound to agonist xanomeline in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ld3
Q-score: 0.518
Draper-Joyce CJ, Bhola R, Wang J, Bhattarai A, Nguyen ATN, Cowie-Kent I, O'Sullivan K, Chia LY, Venugopal H, Valant C, Thal DM, Wootten D, Panel N, Carlsson J, Christie MJ, White PJ, Scammells P, May LT, Sexton PM, Danev R, Miao Y, Glukhova A, Imlach WL, Christopoulos A
Nature (2021) 597 pp. 571-576 [ Pubmed: 34497422 DOI: doi:10.1038/s41586-021-03897-2 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- {2-amino-4-[3,5-bis(trifluoromethyl)phenyl]thiophen-3-yl}(4-chlorophenyl)methanone (449 Da, Ligand)
- Adenosine (267 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-2 (40 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Human adenosine a1 receptor-gi2-protein complex bound to its endogenous agonist adenosine (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Chimera protein of muscarinic acetylcholine receptor m4 and adenosine receptor a1 (43 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ld4
Q-score: 0.502
Draper-Joyce CJ, Bhola R, Wang J, Bhattarai A, Nguyen ATN, Cowie-Kent I, O'Sullivan K, Chia LY, Venugopal H, Valant C, Thal DM, Wootten D, Panel N, Carlsson J, Christie MJ, White PJ, Scammells P, May LT, Sexton PM, Danev R, Miao Y, Glukhova A, Imlach WL, Christopoulos A
Nature (2021) 597 pp. 571-576 [ Pubmed: 34497422 DOI: doi:10.1038/s41586-021-03897-2 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Adenosine (267 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-2 (40 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Human adenosine a1 receptor-gi2-protein complex bound to its endogenous agonist adenosine (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Chimera protein of muscarinic acetylcholine receptor m4 and adenosine receptor a1 (43 kDa, Protein from Homo sapiens)