CRISPR-Cas type III-D effector complex
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8s9t
Q-score: 0.623
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a target RNA
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8s9u
Q-score: 0.584
Schwartz EA, Bravo JPK, Ahsan F, Macias LA, Brodbelt JS, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
To Be Published
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex bound to target rna (351 kDa, Complex from Synechocystis sp. PCC 6803)
- Target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a self-target RNA in the pre-cleavage state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8s9v
Q-score: 0.561
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Self-target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Water (18 Da, Ligand)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Magnesium ion (24 Da, Ligand)
CRISPR-Cas type III-D effector complex bound to self-target RNA in a post-cleavage state
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 8s9x
Q-score: 0.456
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Self-target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex consensus map
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex local refinement map
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
- Crispr-cas type iii-d effector complex (351 kDa, Complex from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a target RNA local refinement map
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
CRISPR-Cas type III-D effector complex bound to a target RNA consensus map
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ Pubmed: 38637512 DOI: doi:10.1038/s41467-024-47506-y ]
Cryo-EM Structure of the Desulfovibrio vulgaris Type I-C Apo Cascade
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 7kha
Q-score: 0.486
O'Brien RE, Santos IC, Wrapp D, Bravo JPK, Schwartz EA, Brodbelt JS, Taylor DW
Nat Commun (2020) 11 pp. 5931-5931 [ DOI: doi:10.1038/s41467-020-19785-8 Pubmed: 33230133 ]
- Crispr-associated protein, tm1801 family (32 kDa, Protein from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303))
- Crispr-associated protein, ct1134 family (25 kDa, Protein from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303))
- Crispr-associated protein, ct1133 family (59 kDa, Protein from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303))
- Rna (45-mer) (14 kDa, RNA from Desulfovibrio vulgaris str. Hildenborough)
- Crispr-associated protein, ct1133 family (14 kDa, Protein from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303))
- Type i-c apo cascade (371 kDa, Complex from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303))