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Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7v3u
Q-score: 0.514
Cheng J, Li N, Huo Y, Dang S, Tye BK, Gao N, Zhai Y
Nat Commun (2022) 13 pp. 1396-1396 [ Pubmed: 35296675 DOI: doi:10.1038/s41467-022-29070-5 ]
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae S288C)
- Mcm double hexamer (dh) (Complex from Saccharomyces cerevisiae S288c)
Cryo-EM structure of MCM double hexamer bound with DDK in State I
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7v3v
Q-score: 0.477
Cheng J, Li N, Huo Y, Dang S, Tye BK, Gao N, Zhai Y
Nat Commun (2022) 13 pp. 1396-1396 [ Pubmed: 35296675 DOI: doi:10.1038/s41467-022-29070-5 ]
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ddk kinase regulatory subunit dbf4 (80 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae S288C)
- Cell division control protein 7 (58 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dh-ddk (state i) (Complex from Saccharomyces cerevisiae S288c)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM map of Dbf4-NTD engaged with Mcm2-NTD-A subtracted from DH-DDK complex
Cryo-EM structure of MCM double hexamer bound with two DDKs (Group I)
Cryo-EM structure of MCM double hexamer bound with two DDKs (Group II)
Cryo-EM structure of MCM double hexamer bound with two DDKs (Group III)
Cryo-EM structure of MCM double hexamer bound with one DDK (Group IV)
Cryo-EM structure of MCM double hexamer bound with one DDK (Group V)
Cryo-EM structure of mutant MCM double hexamer (Mcm4 delta N140) bound with DDK