What is Next-Generation DNA Sequencing

Next-generation sequencing (NGS), also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies including:

  • Illumina (Solexa) sequencing
  • Roche 454 sequencing
  • Ion torrent: Proton / PGM sequencing
  • SOLiD sequencing

These recent technologies allow us to sequence DNA and RNA much more quickly and cheaply than the previously used Sanger sequencing, and as such have revolutionised the study of genomics and molecular biology.

 

Notes

The next few pages will give you an overview of how Illumina, 454 and Ion torrent sequencing work.