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Functional genomics II

Common technologies and data analysis methods

    • Course overview
    • Search within this course
    • Introduction
    • Real-time PCR
    • Microarrays
      • Counteracting dye bias effects when using two-colour arrays
      • Analysis of microarray data
        • Feature extraction
        • Quality control
        • Normalisation
        • Differential expression analysis
        • Biological interpretation of microarray data
        • Submission of data to a public repository
    • What is Next Generation DNA Sequencing?
      • Second-generation sequencing
        • Illumina sequencing
        • 454 sequencing
        • Ion Torrent: Proton / PGM sequencing
      • Third-generation sequencing
        • Nanopore sequencing
        • SMRT sequencing
      • SGS and TGS: test your knowledge
      • Improvements on the previous technology
      • Test your knowledge
    • RNA sequencing
      • Performing a RNA-seq experiment
        • Design considerations
          • Number of replicates
        • Library preparation and sequencing approaches
        • Data analysis
          • Quality control
          • Read mapping or alignment
          • Quantification
          • Differential gene expression analysis
      • Test your knowledge
    • Biological interpretation of gene expression data
    • Genotyping, epigenetic and DNA/RNA-protein interaction methods
      • Epigenetic modifications
        • Histone modifications
    • DNA/RNA-protein interactions
    • Summary
    • Quiz: Check your learning
    • Your feedback
    • Learn more
    • References

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