Performing a RNA-seq experiment

There is no common pipeline for all the different types of RNA-seq. Both the experimental design and the analysis procedures will be different depending on the organism being studied and the research goals. As the primary objective of many biological studies is a comparison of gene expression between samples, we outline a possible workflow for detecting differential expression using RNA-seq (Figure 7).

Overview of a RNA-seq experiment for detecting differential expression
Figure 7 Overview of a RNA-seq experiment for detecting differential expression (DE).