RNA-Seq analysis - Transcriptome assembly and differential expression
In this talk, Myrto Kostadima from the EMBL-EBI discusses how we can use RNA-Seq data to analyse gene expression.
She describes the Cufflinks tool which is a spliced aligner. It can be used to identify different splice variants from RNA-Seq data, and determine their relative frequencies. She discusses how we can use Cufflinks to determine differential gene expression between tissues, individuals or disease states.