Date
Location
Cold Spring Harbor, USA
PDBe Representatives
Gerard Kleywegt - Speaker
X-ray crystallography has been the cornerstone of structural biology for half a century, and remains the technique of choice for atomic resolution understanding of macromolecules and for structure guided drug discovery. This intense course combines laboratory and computational instruction to train course participants in the major techniques used to determine three-dimensional structures. It is designed for scientists with a working knowledge of protein structure and function, but who are new to macromolecular crystallography or who wish to increase their in-depth knowledge of macromolecular crystallography.
Topics to be covered include:
- Basic diffraction theory
- Crystallization (proteins, nucleic acids, complexes and membrane proteins)
- Synchrotron X-ray sources and optics
- Data collection and processing
- Structure solution by experimental phasing methods (SAD, MAD, MIR, and others) and molecular replacement
- Electron density maps improvement
- Model building and refinement
- Structure validation
- Coordinate deposition
- Structure presentation
