Structure analysis

Yeast RQC complex in state H

Electron Microscopy
2.6Å resolution
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero 54-mer (preferred)
Entry contents: 49 distinct polypeptide molecules
4 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 54-mer
Accessible surface area: 766227.27 Å2
Buried surface area: 383494.54 Å2
Dissociation area: 892.04 Å2
Dissociation energy (ΔGdiss): -0.52 kcal/mol
Dissociation entropy (TΔSdiss): 8.49 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-243120

Macromolecules

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Chain: F
Length: 172 amino acids
Theoretical weight: 20.48 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX23 (Residues: 1-172; Coverage: 100%)
Gene names: RPL18A, RPL18A2, RPL20A, YM9408.04C, YMR242C
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
InterPro:

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Chain: H
Length: 121 amino acids
Theoretical weight: 13.71 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05749 (Residues: 1-121; Coverage: 100%)
Gene names: L2168, RPL22A, YLR061W
Pfam: Ribosomal L22e protein family
InterPro:

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Chain: I
Length: 137 amino acids
Theoretical weight: 14.49 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX41 (Residues: 1-137; Coverage: 100%)
Gene names: RPL17A, RPL17AA, RPL23A, YBL0713, YBL087C
Pfam: Ribosomal protein L14p/L23e
InterPro:

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Chain: J
Length: 155 amino acids
Theoretical weight: 17.66 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P04449 (Residues: 1-155; Coverage: 100%)
Gene names: RP29, RPL24A, RPL30A, YGL031C
Pfam: Ribosomal protein L24e
InterPro:

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Chain: O
Length: 59 amino acids
Theoretical weight: 6.69 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05747 (Residues: 1-59; Coverage: 100%)
Gene names: RPL29, YFR032BW, YFR032C-A, YL43
Pfam: Ribosomal L29e protein family
InterPro: Large ribosomal subunit protein eL29

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Chain: Q
Length: 113 amino acids
Theoretical weight: 12.98 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0C2H8 (Residues: 1-113; Coverage: 100%)
Gene names: D2478, RPL31A, RPL34, RPL34A, YDL075W
Pfam: Ribosomal protein L31e
InterPro:

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Chain: R
Length: 130 amino acids
Theoretical weight: 14.81 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38061 (Residues: 1-130; Coverage: 100%)
Gene names: RPL32, YBL0838, YBL092W
Pfam: Ribosomal protein L32
InterPro:

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Chain: T
Length: 121 amino acids
Theoretical weight: 13.67 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P87262 (Residues: 1-121; Coverage: 100%)
Gene names: RPL34A, YER056BC, YER056C-A
Pfam: Ribosomal protein L34e
InterPro:

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Chain: V
Length: 100 amino acids
Theoretical weight: 11.15 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05745 (Residues: 1-100; Coverage: 100%)
Gene names: RPL36A, RPL39A, YM9646.06, YMR194W
Pfam: Ribosomal protein L36e
InterPro:

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Chain: W
Length: 88 amino acids
Theoretical weight: 9.88 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P49166 (Residues: 1-88; Coverage: 100%)
Gene names: L9470.6, RPL35A, RPL37A, YLR185W
Pfam: Ribosomal protein L37e
InterPro:

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Chain: X
Length: 78 amino acids
Theoretical weight: 8.85 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P49167 (Residues: 1-78; Coverage: 100%)
Gene names: L8543.2, RPL38, YLR325C
Pfam: Ribosomal L38e protein family
InterPro:

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Chain: b
Length: 106 amino acids
Theoretical weight: 12.25 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX27 (Residues: 1-106; Coverage: 100%)
Gene names: N1722, RPL41A, RPL42A, SCL41A, YNL162W
Pfam: Ribosomal protein L44
InterPro:

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Chain: c
Length: 92 amino acids
Theoretical weight: 10.11 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX25 (Residues: 1-92; Coverage: 100%)
Gene names: RPL43A, YP9499.02, YPR043W
Pfam: Ribosomal L37ae protein family
InterPro:

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Chain: d
Length: 25 amino acids
Theoretical weight: 3.35 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX86 (Residues: 1-25; Coverage: 100%)
Gene names: D1290, RPL41A, RPL47A, YDL184C, YL41A
Pfam: Ribosomal protein L41
InterPro: Large ribosomal subunit protein eS32

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Chain: m
Length: 297 amino acids
Theoretical weight: 33.76 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P26321 (Residues: 1-297; Coverage: 100%)
Gene names: LPI14W, RPL1, RPL1A, RPL5, YPL131W
Pfam:
InterPro:

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Chain: t
Length: 199 amino acids
Theoretical weight: 22.6 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12690 (Residues: 1-199; Coverage: 100%)
Gene names: RPL13A, YDL082W
Pfam: Ribosomal protein L13e
InterPro:

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Chain: a
Length: 1038 amino acids
Theoretical weight: 119.25 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12532 (Residues: 1-1038; Coverage: 100%)
Gene names: RQC2, TAE2, YP8132.04C, YPL009C
Pfam:
InterPro:

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Chain: g
Length: 245 amino acids
Theoretical weight: 26.48 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12522 (Residues: 1-245; Coverage: 100%)
Gene names: CDC95, LPZ15C, TIF6, YPR016C
Pfam: eIF-6 family
InterPro: Translation initiation factor IF6

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Name: 25S ribosomal RNA
Representative chains: f
Length: 3395 nucleotides
Theoretical weight: 1097.09 KDa
Rfam: Eukaryotic large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: h
Length: 121 nucleotides
Theoretical weight: 38.95 KDa
Rfam: 5S ribosomal RNA

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Name: 5.8S ribosomal RNA
Representative chains: i
Length: 158 nucleotides
Theoretical weight: 50.68 KDa
Rfam: 5.8S ribosomal RNA

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Name: Ala tRNA
Representative chains: x, y
Length: 76 nucleotides
Theoretical weight: 24.5 KDa
Rfam: tRNA

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