DPB1 T-Cell Epitope Algorithm v2.1
Classification of HLA-DPB1 mismatches based on T-cell-epitope groups (TCE-groups) has been shown to identify mismatches that might be better tolerated (permissive) and those that would increase risks (non-permissive) after unrelated-donor haematopoietic stem cell transplantation (HSCT). This calculator allows you to enter the HLA-DPB1 typing of a patient and a donor and retrieve the predicted TCE grouping and resulting matching/mismatching when selecting appropriate donors for HSCT recipients.
Advice on the user of this tool:
- Please provide HLA-DPB1 typing at 2 field resolution, or higher. P groups are also accepted.
- All HLA-DPB1 typing provided should be at the same resolution level (2 field, 3 field, 4 field or P group)
- TCE group assignments between version 2.0 and 2.1 have not changed, however developments in our understanding of immunopeptidome divergence (see Meurer et al. Blood 2021) and the inclusion of bi-directional immunogenicity (see Fleischhauer et al/ BMT 2017) in the algorithm have led to changes in the permissiveness of some mismatch constellations. Version 2.1 and 3 of this tool reflect the current state of the art in terms of TCE assignments and matching.
- For example:
- Patient DPB1*03:01 (TCE 2), DPB1*04:01 (TCE 3) and Donor DPB1*03:01 (TCE 2), DPB1*03:01 (TCE 2)
- In version 2.0 this mismatch would have been predicted as "Permissive", however in version 2.1 this is now predicted as "Non permissive GvH"
- This tool has been developed in collaboration with the original authors and validated with their support.
- No information entered into this tool is collected or stored on our servers.
Disclaimer - This tool is being offered as a tool to predict T-Cell epitope matching at DPB1 as reported in:
References
- Meurer T, Crivello P, Metzing M, et al.
Permissive HLA-DPB1 mismatches in HCT depend on immunopeptidome divergence and editing by HLA-DM.
Blood. (2021) 137:923-928. - Fleischhauer K, Ahn K, Wang H, et al.
Directionality of non-permissive HLA-DPB1 T-cell epitope group mismatches does not improve clinical risk stratification in 8/8 matched unrelated donor hematopoietic cell transplantation.
Bone Marrow Transplant (2017) 52:1280–1287. - Crivello P, Zito L, Sizzano F, et al.
The Impact of Amino Acid Variability on Alloreactivity Defines a Functional Distance Predictive of Permissive HLA-DPB1 Mismatches in Hematopoietic Stem Cell Transplantation
Biol Blood Marrow Transplant (2015) 21:233-41. - Fleischhauer K, Shaw BE, Gooley T, et al.
Effect of T-cell-epitope matching at HLA-DPB1 in recipients of unrelated-donor haemopoietic-cell transplantation: a retrospective study.
Lancet Oncology (2012) 13:366-74 - Crocchiolo R, Zino E, Vago L, et al.
Nonpermissive HLA-DPB1 disparity is a significant independent risk factor for mortality after unrelated hematopoietic stem cell transplantation.
Blood (2009) 114:1437-44. - Zino E, Vago L, Di Terlizzi S, et al.
Frequency and targeted detection of HLA-DPB1 T cell epitope disparities relevant in unrelated hematopoietic stem cell transplantation.
Biol Blood Marrow Transplant (2007) 13:1031-40. - Zino E, Frumento G, Marktel S, et al.
A T-cell epitope encoded by a subset of HLA-DPB1 alleles determines nonpermissive mismatches for hematologic stem cell transplantation.
Blood (2004) 103:1417-24.
No information entered into this tool is collected or stored on our servers.
API
An API has been developed for this tool, please see our API Documentation for further details