PDBsum entry 6tut

Transcription

PDB id: 6tut

Contents

Protein chains

1390 a.a.

1097 a.a.

334 a.a.

104 a.a.

215 a.a.

83 a.a.

199 a.a.

146 a.a.

43 a.a.

67 a.a.

102 a.a.

45 a.a.

166 a.a.

125 a.a.

551 a.a.

196 a.a.

79 a.a.

142 a.a.

Metals

_ZN ×7

PDB id:

6tut

Name:

Transcription

Title:

Cryo-em structure of the RNA polymerase iii-maf1 complex

Structure:

DNA-directed RNA polymerase iii subunit rpc1. Chain: a. Synonym: RNA polymerase iii subunit c1,DNA-directed RNA polymerase iii largest subunit,RNA polymerase iii subunit c160. DNA-directed RNA polymerase iii subunit rpc2. Chain: b. Synonym: RNA polymerase iii subunit c2,c128,DNA-directed RNA polymerase iii 130 kda polypeptide. DNA-directed RNA polymerases i and iii subunit rpac1.

Source:

Saccharomyces cerevisiae s288c. Organism_taxid: 559292. Gene: maf1, ydr005c, yd8119.11c. Expressed in: escherichia coli. Expression_system_taxid: 562

Authors:

M.K.Vorlaender,W.J.H.Hagen,C.W.Mueller

Key ref:

M.K.Vorländer et al. (2020). Structural basis for RNA polymerase III transcription repression by Maf1. Nat Struct Mol Biol, 27, 229-232. PubMed id: 32066962 DOI: 10.1038/s41594-020-0383-y

Date:

08-Jan-20 Release date: 19-Feb-20

Protein chain

P04051  (RPC1_YEAST) -  DNA-directed RNA polymerase III subunit RPC1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1460 a.a.

Struc: 1390 a.a.

Protein chain

P22276  (RPC2_YEAST) -  DNA-directed RNA polymerase III subunit RPC2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1149 a.a.

Struc: 1097 a.a.

Protein chain

P07703  (RPAC1_YEAST) -  DNA-directed RNA polymerases I and III subunit RPAC1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 335 a.a.

Struc: 334 a.a.

Protein chain

P47076  (RPC9_YEAST) -  DNA-directed RNA polymerase III subunit RPC9 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 161 a.a.

Struc: 104 a.a.

Protein chain

P20434  (RPAB1_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 215 a.a.

Struc: 215 a.a.

Protein chain

P20435  (RPAB2_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 155 a.a.

Struc: 83 a.a.

Protein chain

P35718  (RPC8_YEAST) -  DNA-directed RNA polymerase III subunit RPC8 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 212 a.a.

Struc: 199 a.a.

Protein chain

P20436  (RPAB3_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 146 a.a.

Struc: 146 a.a.

Protein chain

Q04307  (RPC10_YEAST) -  DNA-directed RNA polymerase III subunit RPC10 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 110 a.a.

Struc: 43 a.a.

Protein chain

P22139  (RPAB5_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 70 a.a.

Struc: 67 a.a.

Protein chain

P28000  (RPAC2_YEAST) -  DNA-directed RNA polymerases I and III subunit RPAC2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 142 a.a.

Struc: 102 a.a.

Protein chain

P40422  (RPAB4_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 70 a.a.

Struc: 45 a.a.

Protein chain

P36121  (RPC5_YEAST) -  DNA-directed RNA polymerase III subunit RPC5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 282 a.a.

Struc: 166 a.a.

Protein chain

P25441  (RPC4_YEAST) -  DNA-directed RNA polymerase III subunit RPC4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 422 a.a.

Struc: 125 a.a.

Protein chain

P32349  (RPC3_YEAST) -  DNA-directed RNA polymerase III subunit RPC3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 654 a.a.

Struc: 551 a.a.

Protein chain

P32910  (RPC6_YEAST) -  DNA-directed RNA polymerase III subunit RPC6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 317 a.a.

Struc: 196 a.a.

Protein chain

P17890  (RPC7_YEAST) -  DNA-directed RNA polymerase III subunit RPC7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 251 a.a.

Struc: 79 a.a.

Protein chain

P41910  (MAF1_YEAST) -  Repressor of RNA polymerase III transcription MAF1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 395 a.a.

Struc: 142 a.a.*

* PDB and UniProt seqs differ at 27 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1038/s41594-020-0383-y

Nat Struct Mol Biol 27:229-232 (2020)

PubMed id: 32066962

Structural basis for RNA polymerase III transcription repression by Maf1.

M.K.Vorländer, F.Baudin, R.D.Moir, R.Wetzel, W.J.H.Hagen, I.M.Willis, C.W.Müller.

ABSTRACT

Maf1 is a conserved inhibitor of RNA polymerase III (Pol III) that influences phenotypes ranging from metabolic efficiency to lifespan. Here, we present a 3.3-Å-resolution cryo-EM structure of yeast Maf1 bound to Pol III, establishing that Maf1 sequesters Pol III elements involved in transcription initiation and binds the mobile C34 winged helix 2 domain, sealing off the active site. The Maf1 binding site overlaps with that of TFIIIB in the preinitiation complex.