PDBsum entry 4e7x

Transferase

PDB id: 4e7x

Contents

Protein chains

319 a.a.

Ligands

ACT ×4

Waters ×10

PDB id:

4e7x

Name:

Transferase

Title:

Structural basis for the activity of a cytoplasmic RNA terminal u- transferase

Structure:

Poly(a) RNA polymerase protein cid1. Chain: a, b, c, d. Synonym: caffeine-induced death protein 1. Engineered: yes

Source:

Schizosaccharomyces pombe 972h-. Fission yeast. Organism_taxid: 284812. Strain: 972 / atcc 24843. Gene: cid1, spac19d5.03. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

3.20Å R-factor: 0.181 R-free: 0.213

Authors:

L.A.Yates,S.Fleurdepine,O.S.Rissland,L.Decolibus,K.Harlos, C.J.Norbury,R.J.C.Gilbert

Key ref:

L.A.Yates et al. (2012). Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase. Nat Struct Biol, 19, 782-787. PubMed id: 22751018

Date:

19-Mar-12 Release date: 04-Jul-12

Protein chains

O13833  (CID1_SCHPO) -  Terminal uridylyltransferase cid1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: 405 a.a.

Struc: 319 a.a.

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.19 - polynucleotide adenylyltransferase.
• Reaction: RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate
• Enzyme class 2: E.C.2.7.7.52 - Rna uridylyltransferase.
• Reaction: RNA(n) + UTP = RNA(n)-3'-uridine ribonucleotide + diphosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Nat Struct Biol 19:782-787 (2012)

PubMed id: 22751018

Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase.

L.A.Yates, S.Fleurdépine, O.S.Rissland, L.De Colibus, K.Harlos, C.J.Norbury, R.J.Gilbert.

ABSTRACT

No abstract given.