_EC 2.7.7.19 Polynucleotide adenylyltransferase. 21 PDB entries  
EC 2.-.-.- Transferases. [17,949 PDB entries]
EC 2.7.-.- Transferring phosphorous-containing groups. [10,916 PDB entries]
EC 2.7.7.- Nucleotidyltransferases. [4,489 PDB entries]
EC 2.7.7.19 Polynucleotide adenylyltransferase. [21 PDB entries]    
1f5a

Reaction: Atp + RNA(n) = diphosphate + RNA(n+1).
 


ATP
+
RNA(n)
=
diphosphate
+
RNA(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Ntp polymerase. Poly(A) polymerase. Rna adenylating enzyme.
Comments: Also acts slowly with Ctp. Catalyzes template-independent extension of the 3'-end of a Dna strand by one nucleotide at a time. Cannot initiate a chain de novo. The primer, depending on the source of the enzyme, may be an Rna or Dna fragment or oligo(A) bearing a 3'-OH terminal group. See also Ec 2.7.7.6.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 21 PDB entries in enzyme class E.C.2.7.7.19

  PDB code Protein
1f5a
Crystal structure of mammalian poly(a) polymerase
Source: Bos taurus. Cattle. Organism_taxid: 9913. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (463 residues) CATH domains: 3.30.70.590 3.30.460.10 1.10.1410.10
Bound ligands:   Het Group 3AT is 96.00% similar to enzyme reactant ATP
  Het Group 3PO is 69.00% similar to enzyme product diphosphate
1fa0
Structure of yeast poly(a) polymerase bound to manganate and 3'-datp
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (509 residues) CATH domains: 3.30.70.590 3.30.460.10 1.10.1410.10
Bound ligands:   Het Group 3AT is 96.00% similar to enzyme reactant ATP
  Het Group POP corresponds to enzyme product diphosphate
1q78
Crystal structure of poly(a) polymerase in complex with 3'- datp and magnesium chloride
Source: Bos taurus. Cattle. Organism_taxid: 9913. Gene: papola or pap. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (470 residues) CATH domains: 3.30.70.590 3.30.460.10 1.10.1410.10
Bound ligand:   Het Group 3AT is 96.00% similar to enzyme reactant ATP
1q79
Crystal structure of mammalian poly(a) polymerase
Source: Bos taurus. Cattle. Organism_taxid: 9913. Gene: papola or pap. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (470 residues) CATH domains: 3.30.70.590 3.30.460.10 1.10.1410.10
Bound ligand:   Het Group 3AT is 96.77% similar to enzyme reactant ATP
2e5g
Solution structure of RNA binding domain in RNA binding motif protein 21
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: tut1, rbm21. Other_details: cell-free protein synthesis
Chain: A (94 residues) CATH domain: 3.30.70.330
2ga9
Crystal structure of the heterodimeric vaccinia virus polyad polymerase with bound atp-gamma-s
Source: Vaccinia virus. Organism_taxid: 10245. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: D (445 residues) CATH domains: Unassigned Unassigned Unassigned
Bound ligand:   Het Group AGS is 93.75% similar to enzyme reactant ATP
2gaf
Crystal structure of the vaccinia polyadenylate polymerase heterodimer (apo form)
Source: Vaccinia virus. Organism_taxid: 10245. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: D (447 residues) CATH domains: Unassigned Unassigned Unassigned
2hhp
Structure of yeast poly(a) polymerase in a closed conformati
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: pap1. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (508 residues) CATH domains: 1.10.1410.10 3.30.460.10 3.30.70.590
2o1p
Structure of yeast poly(a) polymerase in a somewhat closed s
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: pap1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (523 residues) CATH domains: 1.10.1410.10 3.30.460.10 3.30.70.590
2q66
Structure of yeast poly(a) polymerase with atp and oligo(a)
Source: Synthetic: yes. Other_details: synthetic. Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: pap1. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (519 residues) CATH domains: 1.10.1410.10 3.30.460.10 3.30.70.590
Bound ligand:   Het Group ATP corresponds to enzyme reactant ATP
3aqk
Structure of bacterial protein (apo form i)
Source: Escherichia coli. Organism_taxid: 536056. Strain: k12. Gene: ecdh1_3459. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (390 residues) CATH domain: 3.30.460.10
3aql
Structure of bacterial protein (apo form ii)
Source: Escherichia coli. Organism_taxid: 536056. Strain: k12. Gene: ecdh1_3459. Expressed in: escherichia coli. Expression_system_taxid: 536056.
Chains: A, B (388 residues) CATH domain: 3.30.460.10
3aqm
Structure of bacterial protein (form ii)
Source: Escherichia coli. Organism_taxid: 536056. Strain: k12. Gene: ecdh1_3459. Expressed in: escherichia coli. Expression_system_taxid: 536056.
Chains: A, B (388 residues) CATH domain: 3.30.460.10
3aqn
Complex structure of bacterial protein (apo form ii)
Source: Escherichia coli. Organism_taxid: 536056. Strain: k12. Gene: ecdh1_3459. Expressed in: escherichia coli. Expression_system_taxid: 536056.
Chains: A, B (388 residues) CATH domain: 3.30.460.10
Bound ligand:   Het Group ATP corresponds to enzyme reactant ATP
3c66
Yeast poly(a) polymerase in complex with fip1 residues 80-10
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: pap1, ykr002w. Expressed in: escherichia coli. Synthetic: yes
Chains: A, B (529 residues) CATH domains: 1.10.1410.10 3.30.460.10 3.30.70.590
3er8
Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with two fragments of RNA
Source: Vaccinia virus wr. Organism_taxid: 10254. Strain: western reserve / wr. Gene: paps, vacwr095, f9. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: papl, vacwr057, e1l. Synthetic: yes. Synthetic: yes
Chains: C, D (445 residues) CATH domains: Unassigned Unassigned Unassigned
3er9
Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with uu and 3'-deoxy atp
Source: Vaccinia virus wr. Organism_taxid: 10254. Strain: western reserve / wr. Gene: paps, vacwr095, f9. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: papl, vacwr057, e1l. Synthetic: yes
Chain: B (445 residues) CATH domains: Unassigned Unassigned Unassigned
Bound ligand:   Het Group 3AT is 96.77% similar to enzyme reactant ATP
3erc
Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase with three fragments of RNA and 3'-deoxy atp
Source: Vaccinia virus wr. Organism_taxid: 10254. Strain: western reserve / wr. Gene: paps, vacwr095, f9. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: papl, vacwr057, e1l. Synthetic: yes. Synthetic: yes
Chains: C, D (445 residues) CATH domains: Unassigned Unassigned Unassigned
Bound ligand:   Het Group 3AT is 96.00% similar to enzyme reactant ATP
3owg
Crystal structure of vaccinia virus polyadenylate polymerase
Source: Vaccinia virus wr. Vacv. Organism_taxid: 10254. Strain: western reserve. Gene: papl, vacwr057, e1l. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (457 residues) CATH domains: Unassigned Unassigned Unassigned
3pq1
Crystal structure of human mitochondrial poly(a) polymerase
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: mtpap, papd1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (355 residues) CATH domain: Unassigned
4lt6
Crystal structure of human poly(a) polymerase gamma
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: papolg, pap2, papg. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (467 residues) CATH domains: 1.10.1410.10 3.30.460.10 3.30.70.590
Bound ligand:   Het Group 3AT is 96.77% similar to enzyme reactant ATP