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UniProt functional annotation for O13833 | ||
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| UniProt code: O13833. |
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| Organism: | |
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. |
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| Function: | |
Cytoplasmic uridylyltransferase that mediates the terminal uridylation of mRNAs with short poly(A) tails such as such as act1, hcn1 and urg1 mRNAs, hence facilitating global mRNA decay (PubMed:17353264, PubMed:17449726, PubMed:19430462, PubMed:22751018, PubMed:22885303). Uridylates the 3' ends of actin mRNAs upon S-phase arrest (PubMed:17353264). Has also a weak poly(A) polymerase (PAP) activity (PubMed:22751018, PubMed:22885303). Residue His-336 is responsible for the specificity for UTP (PubMed:22751018, PubMed:22885303). Involved in cell cycle arrest where in association with crb2/rhp9 and chk1 it inhibits unscheduled mitosis (PubMed:10757807). {ECO:0000269|PubMed:10757807, ECO:0000269|PubMed:17353264, ECO:0000269|PubMed:17449726, ECO:0000269|PubMed:22751018, ECO:0000269|PubMed:22885303}. |
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| Catalytic activity: | |
Reaction=RNA(n) + UTP = diphosphate + RNA(n)-3'-uridine ribonucleotide; Xref=Rhea:RHEA:14785, Rhea:RHEA-COMP:11128, Rhea:RHEA-COMP:14648, ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:83400, ChEBI:CHEBI:140627; EC=2.7.7.52; Evidence={ECO:0000269|PubMed:17353264, ECO:0000269|PubMed:17449726, ECO:0000269|PubMed:19430462, ECO:0000269|PubMed:22751018, ECO:0000269|PubMed:22885303, ECO:0000269|PubMed:24322298, ECO:0000269|PubMed:25712096}; |
| Catalytic activity: | |
Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide; Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:11128, Rhea:RHEA-COMP:14647, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:83400, ChEBI:CHEBI:140626; EC=2.7.7.19; Evidence={ECO:0000269|PubMed:12218190, ECO:0000269|PubMed:22751018, ECO:0000269|PubMed:22885303}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17353264, ECO:0000269|PubMed:22885303}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:17353264}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=12 uM for UTP {ECO:0000269|PubMed:22885303}; KM=312 uM for ATP {ECO:0000269|PubMed:22885303}; Note=Mutation of His-336 to Asn decreases the Km for ATP to 65 uM and increases the Km for UTP to 45 uM. {ECO:0000269|PubMed:22885303}; |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:12218190}. |
| Disruption phenotype: | |
Stabilizes urg1 transcripts (PubMed:19430462). {ECO:0000269|PubMed:19430462}. |
| Similarity: | |
Belongs to the DNA polymerase type-B-like family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.