› 8g99

Electron Microscopy
2.8Å resolution

Partial auto-inhibitory complex of Xenopus laevis DNA polymerase alpha-primase

Released:
DOI: 10.2210/pdb8g99/pdb
PDB entry 8g99 coloured by chain, front view.
PDB entry 8g99 coloured by chain, side view.
PDB entry 8g99 coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
A mechanistic model of primer synthesis from catalytic structures of DNA polymerase α-primase.
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024)
PMID: 38491139
Related structures: EMD-29862

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-199714 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA polymerase alpha catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 1127 amino acids
Theoretical weight: 129.01 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9DE46 (Residues: 335-1458; Coverage: 77%)
Gene names: pola, pola1
Sequence domains:
DNA polymerase alpha subunit B Chain: B
Molecule details ›
Chain: B
Length: 601 amino acids
Theoretical weight: 67.19 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6DCZ1 (Residues: 1-598; Coverage: 100%)
Gene names: MGC80532, pola2, pola2.L
Sequence domains:
DNA primase large subunit Chain: C
Molecule details ›
Chain: C
Length: 513 amino acids
Theoretical weight: 59.67 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1L8G3G3 (Residues: 1-513; Coverage: 100%)
Gene name: prim2.S
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-29862
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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