› 7c0w

X-ray diffraction
2.1Å resolution

Crystal structure of a dinucleotide-binding protein (Y224A) of ABC transporter endogenously bound to uridylyl-3'-5'-phospho-guanosine (Form II)

Released:
DOI: 10.2210/pdb7c0w/pdb
PDB entry 7c0w coloured by chain, front view.
PDB entry 7c0w coloured by chain, side view.
PDB entry 7c0w coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structural and thermodynamic insights into the novel dinucleotide-binding protein of ABC transporter unveils its moonlighting function.
Chandravanshi M, Samanta R, Kanaujia SP
FEBS J 288 4614-4636 (2021)
PMID: 33599038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178146 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sugar ABC transporter, periplasmic sugar-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 44.41 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLB4 (Residues: 20-417; Coverage: 100%)
Gene name: TTHA0379
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

5 bound ligands:
Ligand NA 1 x NA
Ligand CO2 2 x CO2
Ligand EDO 1 x EDO
Ligand GOL 2 x GOL
Ligand FGO 2 x FGO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 65.96Å b: 58.06Å c: 103.08Å
α: 90° β: 93.25° γ: 90°
R-values:
R R work R free
0.203 0.201 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

A genome-wide association study reveals that epistasis underlies the pathogenicity of Pectobacterium.
Chen et al. (2023)
1 more

1 mention without citation

Structural and thermodynamic insights into the novel dinucleotide-binding protein of ABC transporter unveils its moonlighting function.
Chandravanshi et al. (2021)