› 5tp4

X-ray diffraction
1.7Å resolution

Crystal structure of a hydantoinase/carbamoylase family amidase from Burkholderia ambifaria

Released:
DOI: 10.2210/pdb5tp4/pdb
PDB entry 5tp4 coloured by chain, front view.
PDB entry 5tp4 coloured by chain, side view.
PDB entry 5tp4 coloured by chain, top view.
Source organism: Burkholderia ambifaria MC40-6
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H(2)O = L-2-amino acid + NH(3) + CO(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidase, hydantoinase/carbamoylase family Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 45.47 KDa
Source organism: Burkholderia ambifaria MC40-6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1YW99 (Residues: 1-423; Coverage: 100%)
Gene name: BamMC406_1144
Sequence domains: Peptidase family M20/M25/M40
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand MES 2 x MES
Ligand EDO 10 x EDO
Ligand FMT 7 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 42.53Å b: 136.96Å c: 164.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.191
Expression system: Escherichia coli BL21(DE3)

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