Peptidase M20 (IPR002933)

Short name: Peptidase_M20

Overlapping homologous superfamilies

Family relationships


This group of proteins contains the metallopeptidases and non-peptidase homologues (amidohydrolases) that belong to the MEROPS peptidase family M20 (clan MH) [PMID: 7674922]. The peptidases of this clan have two catalytic zinc ions at the active site, bound by His/Asp, Asp, Glu, Asp/Glu and His. The catalysed reaction involves the release of an N-terminal amino acid, usually neutral or hydrophobic, from a polypeptide [PMID: 7674922]. The peptidase M20 family has four subfamilies:

  • M20A - type example, glutamate carboxypeptidase from Pseudomonas sp. RS16 (P06621)
  • M20B - type example, peptidase T from Escherichia coli (P29745)
  • M20C - type example, X-His dipeptidase from E. coli (P15288)
  • M20D - type example, carboxypeptidase Ss1 from Sulfolobus solfataricus (P80092)

Homologues from the family that are not peptidases include:

  • Acetylornithine deacetylase, which releases an acetyl group and L-ornithine from N(2)-acetyl-L-ornithine [PMID: 10684608]
  • N-acetyldiaminopimelate deacetylase, which catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate [PMID: 5411754]
  • N-carbamoyl-L-amino-acid hydrolase, which converts D,L-5-monosubstituted hydantoins into D- or L-amino acids [PMID: 1732229]
  • Aminoacylase-1, which hydrolyses N-acylated or N-acetylated amino acids [PMID: 12933810]
  • Succinyl-diaminopimelate desuccinylase (EC:, which catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid, forming succinate and LL-2,6-diaminoheptanedioate (DAP, a component of bacterial cell walls) [PMID: 3276674]

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0016787 hydrolase activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.