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Polymerase-damaged DNA complex

X-ray diffraction
2.45Å resolution
PDB entry 4q43 coloured by chain, front view.
PDB entry 4q43 coloured by chain, side view.
PDB entry 4q43 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA polymerase IV and DNA
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Multimeric state: hetero trimer
Accessible surface area: 21473.04 Å2
Buried surface area: 4923.59 Å2
Dissociation area: 1,291.52 Å2
Dissociation energy (ΔGdiss): 43.97 kcal/mol
Dissociation entropy (TΔSdiss): 11.21 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-118003
Assembly 2
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Assembly Name: DNA polymerase IV and DNA
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Multimeric state: hetero trimer
Accessible surface area: 20282.86 Å2
Buried surface area: 4905.94 Å2
Dissociation area: 1,323.61 Å2
Dissociation energy (ΔGdiss): 44.8 kcal/mol
Dissociation entropy (TΔSdiss): 10.78 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-118003

Macromolecules

DNA polymerase IV
Chains: A, F
Length: 352 amino acids
Theoretical weight: 39.59 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47155 (Residues: 2-351; Coverage: 100%)
Gene names: JW0221, b0231, dinB, dinP
Pfam:
InterPro:
CATH:
DNA polymerase IV in PDB entry 4q43, assembly 1, front view.
DNA polymerase IV in PDB entry 4q43, assembly 1, side view.
DNA polymerase IV in PDB entry 4q43, assembly 1, top view.
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DNA chains B, G
Name: DNA (5'-D(*TP*CP*TP*(RDG)P*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3')
Representative chains: B, G
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 18 nucleotides
Theoretical weight: 5.59 KDa
DNA (5'-D(*TP*CP*TP*(RDG)P*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') in PDB entry 4q43, assembly 1, front view.
DNA (5'-D(*TP*CP*TP*(RDG)P*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') in PDB entry 4q43, assembly 1, side view.
DNA (5'-D(*TP*CP*TP*(RDG)P*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') in PDB entry 4q43, assembly 1, top view.
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DNA chains C, H
Name: DNA (5'-D(*T*CP*TP*AP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3')
Representative chains: C, H
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
DNA (5'-D(*T*CP*TP*AP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') in PDB entry 4q43, assembly 1, front view.
DNA (5'-D(*T*CP*TP*AP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') in PDB entry 4q43, assembly 1, side view.
DNA (5'-D(*T*CP*TP*AP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') in PDB entry 4q43, assembly 1, top view.
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