› 4n3n

X-ray diffraction
2.75Å resolution

Crystal structure of eukaryotic translation initiation factor eIF5B (517-1116) from Chaetomium thermophilum, apo form

Released:
DOI: 10.2210/pdb4n3n/pdb
PDB entry 4n3n coloured by chain, front view.
PDB entry 4n3n coloured by chain, side view.
PDB entry 4n3n coloured by chain, top view.
Source organism: Thermochaetoides thermophila DSM 1495
Primary publication:
eIF5B employs a novel domain release mechanism to catalyze ribosomal subunit joining.
Kuhle B, Ficner R
EMBO J 33 1177-91 (2014)
PMID: 24686316

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-124042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 5B Chain: A
Molecule details ›
Chain: A
Length: 603 amino acids
Theoretical weight: 67.24 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0S8G9 (Residues: 517-575, 600-1116; Coverage: 52%)
Gene name: CTHT_0029840
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LAC 1 x LAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P3221
Unit cell:
a: 111.47Å b: 111.47Å c: 115.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Protein Synthesis Initiation in Eukaryotic Cells.
Merrick et al. (2018)
7 more

3 mentions without citation

Recent Advances in Archaeal Translation Initiation.
Schmitt et al. (2020)
2 more