› 4f66

X-ray diffraction
1.48Å resolution

The crystal structure of 6-phospho-beta-glucosidase from Streptococcus mutans UA159 in complex with beta-D-glucose-6-phosphate.

Released:
DOI: 10.2210/pdb4f66/pdb
PDB entry 4f66 coloured by chain, front view.
PDB entry 4f66 coloured by chain, side view.
PDB entry 4f66 coloured by chain, top view.
Source organism: Streptococcus mutans
Primary publication:
GH1-family 6-P-β-glucosidases from human microbiome lactic acid bacteria.
Michalska K, Tan K, Li H, Hatzos-Skintges C, Bearden J, Babnigg G, Joachimiak A
OpenAccess logo Acta Crystallogr D Biol Crystallogr 69 451-63 (2013)
PMID: 23519420

Function and Biology Details

Reaction catalysed: 
6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-beta-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 480 amino acids
Theoretical weight: 55.48 KDa
Source organism: Streptococcus mutans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DT00 (Residues: 1-477; Coverage: 100%)
Gene names: SMU_1601, bgl
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand BG6 2 x BG6
Ligand EDO 4 x EDO
Ligand FMT 2 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 58.665Å b: 92.449Å c: 94.351Å
α: 90° β: 101.32° γ: 90°
R-values:
R R work R free
0.163 0.162 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

β-Glucosidase activities of lactic acid bacteria: mechanisms, impact on fermented food and human health.
Michlmayr et al. (2014)
1 more

2 mentions without citation

The cytochrome P450 genes of channel catfish: their involvement in disease defense responses as revealed by meta-analysis of RNA-Seq data sets.
Zhang et al. (2014)
1 more