Active Site

Glycoside hydrolase family 1, active site (IPR018120)

Short name: Glyco_hydro_1_AS

Description

Glycoside hydrolase family 1 GH1 comprises enzymes with a number of known activities; beta-glucosidase (EC:3.2.1.21); beta-galactosidase (EC:3.2.1.23); 6-phospho-beta-galactosidase (EC:3.2.1.85); 6-phospho-beta-glucosidase (EC:3.2.1.86); lactase-phlorizin hydrolase (EC:3.2.1.62)/(EC:3.2.1.108); beta-mannosidase (EC:3.2.1.25); myrosinase (EC:3.2.1.147).

This entry represents a conserved region found in these enzymes. It is centred on a conserved glutamic acid residue which has been shown in the beta-glucosidase from Agrobacterium, to be directly involved in glycosidic bond cleavage by acting as a nucleophile.

Signature in this entry also picks up the last two domains of LPH (lactase-phlorizin hydrolase); the first two domains, which are removed from the LPH precursor by proteolytic processing, have lost the active site glutamate and may therefore be inactive [PMID: 1732212].

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE patterns