› 4c3l

X-ray diffraction
1.6Å resolution

Structure of wildtype PII from S. elongatus at high resolution

Released:
DOI: 10.2210/pdb4c3l/pdb
PDB entry 4c3l coloured by chain, front view.
PDB entry 4c3l coloured by chain, side view.
PDB entry 4c3l coloured by chain, top view.
Source organism: Synechococcus elongatus
Primary publication:
Structural basis and target-specific modulation of ADP sensing by the Synechococcus elongatus PII signaling protein.
Zeth K, Fokina O, Forchhammer K
J Biol Chem 289 8960-72 (2014)
PMID: 24519945

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein P-II Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.41 KDa
Source organism: Synechococcus elongatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3F4 (Residues: 1-112; Coverage: 100%)
Gene names: Synpcc7942_0321, glnB
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P321
Unit cell:
a: 65.693Å b: 65.693Å c: 49.202Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite.
Huergo et al. (2015)
10 more

1 mention without citation

Structural basis and target-specific modulation of ADP sensing by the Synechococcus elongatus PII signaling protein.
Zeth et al. (2014)