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Structure of phosphofructokinase from Saccharomyces cerevisiae

X-ray diffraction
2.9Å resolution
PDB entry 3o8o coloured by chain, front view.
PDB entry 3o8o coloured by chain, side view.
PDB entry 3o8o coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: ATP-dependent 6-phosphofructokinase
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Multimeric state: hetero tetramer
Accessible surface area: 100204.95 Å2
Buried surface area: 16781.18 Å2
Dissociation area: 1,047.85 Å2
Dissociation energy (ΔGdiss): 2.27 kcal/mol
Dissociation entropy (TΔSdiss): 16.95 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-147822
Assembly 2
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Assembly Name: ATP-dependent 6-phosphofructokinase
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Multimeric state: hetero tetramer
Accessible surface area: 100841.07 Å2
Buried surface area: 16421.12 Å2
Dissociation area: 1,108.89 Å2
Dissociation energy (ΔGdiss): 3.59 kcal/mol
Dissociation entropy (TΔSdiss): 16.97 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-147822

Macromolecules

ATP-dependent 6-phosphofructokinase subunit alpha
Chains: A, C, E, G
Length: 787 amino acids
Theoretical weight: 86 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P16861 (Residues: 201-987; Coverage: 80%)
Gene names: G8599, PFK1, YGR240C
Pfam: Phosphofructokinase
InterPro:
CATH:
ATP-dependent 6-phosphofructokinase subunit alpha in PDB entry 3o8o, assembly 1, front view.
ATP-dependent 6-phosphofructokinase subunit alpha in PDB entry 3o8o, assembly 1, side view.
ATP-dependent 6-phosphofructokinase subunit alpha in PDB entry 3o8o, assembly 1, top view.
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ATP-dependent 6-phosphofructokinase subunit beta
Chains: B, D, F, H
Length: 766 amino acids
Theoretical weight: 83.54 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P16862 (Residues: 194-959; Coverage: 80%)
Gene names: PFK2, YM8325.06C, YMR205C
Pfam: Phosphofructokinase
InterPro:
CATH:
ATP-dependent 6-phosphofructokinase subunit beta in PDB entry 3o8o, assembly 1, front view.
ATP-dependent 6-phosphofructokinase subunit beta in PDB entry 3o8o, assembly 1, side view.
ATP-dependent 6-phosphofructokinase subunit beta in PDB entry 3o8o, assembly 1, top view.
Molecule details ›

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