› 3hj6

X-ray diffraction
2.8Å resolution

Structure of Halothermothrix orenii fructokinase (FRK)

Released:
DOI: 10.2210/pdb3hj6/pdb
PDB entry 3hj6 coloured by chain, front view.
PDB entry 3hj6 coloured by chain, side view.
PDB entry 3hj6 coloured by chain, top view.
Source organism: Halothermothrix orenii H 168
Primary publication:
Crystal structure of a fructokinase homolog from Halothermothrix orenii.
Chua TK, Seetharaman J, Kasprzak JM, Ng C, Patel BK, Love C, Bujnicki JM, Sivaraman J
J Struct Biol 171 397-401 (2010)
PMID: 20493950

Function and Biology Details

Reaction catalysed: 
ATP + D-fructose = ADP + D-fructose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 36.26 KDa
Source organism: Halothermothrix orenii H 168
Expression system: Escherichia coli
UniProt:
  • Canonical: B8CZ52 (Residues: 1-320; Coverage: 100%)
Gene name: Hore_18220
Sequence domains: pfkB family carbohydrate kinase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 43.446Å b: 171.941Å c: 46.598Å
α: 90° β: 112.97° γ: 90°
R-values:
R R work R free
0.254 0.254 0.288
Expression system: Escherichia coli

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Citations

1 review citation

Review of the enzymatic machinery of Halothermothrix orenii with special reference to industrial applications.
Bhattacharya et al. (2014)
7 other articles

2 mentions without citation

Plant Fructokinases: Evolutionary, Developmental, and Metabolic Aspects in Sink Tissues.
Stein et al. (2018)
1 more