PDBe 3nuh

X-ray diffraction
3.1Å resolution

A domain insertion in E. coli GyrB adopts a novel fold that plays a critical role in gyrase function

Released:

Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA gyrase subunit A Chain: A
Molecule details ›
Chain: A
Length: 525 amino acids
Theoretical weight: 59.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AES4 (Residues: 1-525; Coverage: 60%)
Gene names: JW2225, b2231, gyrA, hisW, nalA, parD
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
Structure domains:
DNA gyrase subunit B Chain: B
Molecule details ›
Chain: B
Length: 420 amino acids
Theoretical weight: 48.23 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AES6 (Residues: 389-804; Coverage: 52%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2221
Unit cell:
a: 108.062Å b: 147.493Å c: 138.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.234 0.282
Expression system: Escherichia coli BL21