DNA topoisomerase, type IIA-like domain superfamily (IPR013760)
Short name: Topo_IIA-like_dom_sf
- DNA topoisomerase II, eukaryotic-type (IPR001154)
- DNA topoisomerase, type IIA, subunit A/C-terminal (IPR002205)
- DNA gyrase B subunit, C-terminal (IPR002288)
- DNA topoisomerase IV subunit A, Gram-positive (IPR005741)
- DNA topoisomerase IV, subunit A, Gram-negative (IPR005742)
- DNA gyrase, subunit A (IPR005743)
- Type IIA DNA topoisomerase subunit A, alpha-helical domain superfamily (IPR013757)
- DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta (IPR013758)
- DNA topoisomerase, type IIA, subunit B, C-terminal (IPR013759)
- DNA topoisomerase, type IIA, conserved site (IPR018522)
- C-terminal associated domain of TOPRIM (IPR031660)
- DNA topoisomerase 2, TOPRIM domain (IPR034157)
- DNA gyrase subunit B, TOPRIM domain (IPR034160)
Type IIA topoisomerases together manage chromosome integrity and topology in cells. Topoisomerase II (called gyrase in bacteria) primarily introduces negative supercoils into DNA. In bacteria, topoisomerase II consists of two polypeptide subunits, gyrA and gyrB, which form a heterotetramer: (BA)2. In most eukaryotes, topoisomerase II consists of a single polypeptide, where the N- and C-terminal regions correspond to gyrB and gyrA, respectively; this topoisomerase II forms a homodimer that is equivalent to the bacterial heterotetramer. There are four functional domains in topoisomerase II: domain 1 (N-terminal of gyrB) is an ATPase, domain 2 (C-terminal of gyrB) is responsible for subunit interactions (differs between eukaryotic and bacterial enzymes), domain 3 (N-terminal of gyrA) is responsible for the breaking-rejoining function through its capacity to form protein-DNA bridges, and domain 4 (C-terminal of gyrA) is able to non-specifically bind DNA [PMID: 8982450].
Topoisomerase IV primarily decatenates DNA and relaxes positive supercoils, which is important in bacteria, where the circular chromosome becomes catenated, or linked, during replication [PMID: 16023670]. Topoisomerase IV consists of two polypeptide subunits, parE and parC, where parC is homologous to gyrA and parE is homologous to gyrB.
This superfamily represents the C-terminal of subunit B (gyrB and parE) and the N-terminal of subunit A (gyrA and parC) of bacterial gyrase and topoisomerase IV, and the equivalent region in eukaryotic topoisomerase II composed of a single polypeptide.
- SSF56719 (SSF56719)