› 2v6v

X-ray diffraction
1.5Å resolution

The structure of the Bem1p PX domain

Released:
DOI: 10.2210/pdb2v6v/pdb
PDB entry 2v6v coloured by chain, front view.
PDB entry 2v6v coloured by chain, side view.
PDB entry 2v6v coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural and membrane binding analysis of the Phox homology domain of Bem1p: basis of phosphatidylinositol 4-phosphate specificity.
Stahelin RV, Karathanassis D, Murray D, Williams RL, Cho W
J Biol Chem 282 25737-47 (2007)
PMID: 17581820

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bud emergence protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 18.16 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P29366 (Residues: 266-413; Coverage: 27%)
Gene names: BEM1, SRO1, YBR1412, YBR200W
Sequence domains: PX domain
Structure domains: Phox-like domain

Ligands and Environments

1 bound ligand:
Ligand DTT 1 x DTT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 61.015Å b: 71.621Å c: 75.316Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.256
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Conditional peripheral membrane proteins: facing up to limited specificity.
Moravcevic et al. (2012)
9 more

1 mention without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)