PDBe 2j6f

X-ray diffraction
1.7Å resolution

N-TERMINAL SH3 DOMAIN OF CMS (CD2AP HUMAN HOMOLOG) BOUND TO CBL-B PEPTIDE

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CD2-associated protein Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 7.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5K6 (Residues: 1-62; Coverage: 10%)
Gene name: CD2AP
Sequence domains: Variant SH3 domain
Structure domains: SH3 Domains
E3 ubiquitin-protein ligase CBL-B Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13191 (Residues: 902-912; Coverage: 1%)
Gene names: CBLB, Nbla00127, RNF56

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: I422
Unit cell:
a: 66.51Å b: 66.51Å c: 67.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.218
Expression systems:
  • Escherichia coli
  • Not provided