Function and Biology

N-TERMINAL SH3 DOMAIN OF CMS (CD2AP HUMAN HOMOLOG) BOUND TO CBL-B PEPTIDE

Source organism: Homo sapiens
Biochemical function: not assigned
Biological process: not assigned
Cellular component: not assigned

EC 2.3.2.27: RING-type E3 ubiquitin transferase

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
Comments:
  • The RING domain of E3 ubiquitin transferase serves as a mediator bringing the ubiquitin-charged E2 ubiquitin-conjugating enzyme and the acceptor protein together to enable the direct transfer of ubiquitin through the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin an the epsilon-amino group of an L-lysine residue of the acceptor protein.
  • The RING-E3 domain does not form a catalytic thioester intermediate with ubiquitin (unlike the HECT domain, EC 2.3.2.26).
  • RING-type ubiquitin transferases may occur as single-chain enzymes but also in dimeric forms or in multi-subunit assemblies.
  • Formerly EC 6.3.2.19 and EC 6.3.2.21.
Systematic name:
S-ubiquitinyl-[ubiquitin-conjugating E2 enzyme]-L-cysteine:acceptor protein ubiquitin transferase (isopeptide bond-forming; RING-type)
Alternative Name(s):
  • RING E3 ligase
  • Ubiquitin transferase RING E3

GO terms

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Sequence family

Pfam Protein family (Pfam)
PF14604
Domain description: Variant SH3 domain
Occurring in:
  1. CD2-associated protein
1 copy of Pfam domain PF14604 (Variant SH3 domain) in CD2-associated protein in PDB 2j6f.

InterPro InterPro annotations
IPR001452
Domain description: SH3 domain
Occurring in:
  1. CD2-associated protein
IPR035775
Domain description: CD2-associated protein, first SH3 domain
Occurring in:
  1. CD2-associated protein
IPR036028
Domain description: SH3-like domain superfamily
Occurring in:
  1. CD2-associated protein
IPR028445
Domain description: CD2-associated protein
Occurring in:
  1. CD2-associated protein

Structure domain

CATH CATH domain
2.30.30.40
Class: Mainly Beta
Architecture: Roll
Topology: SH3 type barrels.
Homology: SH3 Domains
Occurring in:
  1. CD2-associated protein
1 copy of CATH domain 2.30.30.40 (SH3 type barrels.) in CD2-associated protein in PDB 2j6f.