› 5hbo

X-ray diffraction
1.66Å resolution

Native rhodanese domain of YgaP prepared without DDT is both S-nitrosylated and S-sulfhydrated

Released:
DOI: 10.2210/pdb5hbo/pdb
PDB entry 5hbo coloured by chain, front view.
PDB entry 5hbo coloured by chain, side view.
PDB entry 5hbo coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
S-Nitrosylation Induces Structural and Dynamical Changes in a Rhodanese Family Protein.
Eichmann C, Tzitzilonis C, Nakamura T, Kwiatkowski W, Maslennikov I, Choe S, Lipton SA, Riek R
J Mol Biol 428 3737-51 (2016)
PMID: 27473602

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inner membrane protein YgaP Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 14.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55734 (Residues: 2-109; Coverage: 62%)
Gene names: JW2643, b2668, ygaP
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

No bound ligands
2 modified residues:
Ligand CSS 1 x CSS
Ligand SNC 1 x SNC

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P31
Unit cell:
a: 43.683Å b: 43.683Å c: 52.417Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.176 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Computational Structural Biology of S-nitrosylation of Cancer Targets.
Bignon et al. (2018)
5 other articles

2 mentions without citation

S-Nitrosylation Induces Structural and Dynamical Changes in a Rhodanese Family Protein.
Eichmann et al. (2016)
1 more