› 5f51

X-ray diffraction
2.53Å resolution

Structure of B. abortus WrbA-related protein A (apo)

Released:
DOI: 10.2210/pdb5f51/pdb
PDB entry 5f51 coloured by chain, front view.
PDB entry 5f51 coloured by chain, side view.
PDB entry 5f51 coloured by chain, top view.
Source organism: Brucella abortus 2308
Primary publication:
WrpA Is an Atypical Flavodoxin Family Protein under Regulatory Control of the Brucella abortus General Stress Response System.
Herrou J, Czyż DM, Willett JW, Kim HS, Chhor G, Babnigg G, Kim Y, Crosson S
J Bacteriol 198 1281-93 (2016)
PMID: 26858101

Function and Biology Details

Reaction catalysed: 
NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)H dehydrogenase (quinone) Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.64 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q2YQ23 (Residues: 1-199; Coverage: 100%)
Gene name: BAB1_1070
Sequence domains: NADPH-dependent FMN reductase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P4222
Unit cell:
a: 61.28Å b: 61.28Å c: 128.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.239 0.268
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

9 citation in other articles

Brucella abortus Induces a Warburg Shift in Host Metabolism That Is Linked to Enhanced Intracellular Survival of the Pathogen.
Czyż et al. (2017)
8 more

3 mentions without citation

WrpA Is an Atypical Flavodoxin Family Protein under Regulatory Control of the Brucella abortus General Stress Response System.
Herrou et al. (2016)
2 more