› 4psr

X-ray diffraction
1.38Å resolution

Crystal Structure of alpha-L-fucosidase from Fusarium graminearum in the open form in complex with L-fucose

Released:
DOI: 10.2210/pdb4psr/pdb
PDB entry 4psr coloured by chain, front view.
PDB entry 4psr coloured by chain, side view.
PDB entry 4psr coloured by chain, top view.
Source organism: Fusarium graminearum
Primary publication:
Structure and substrate specificity of a eukaryotic fucosidase from Fusarium graminearum.
Cao H, Walton JD, Brumm P, Phillips GN
J Biol Chem 289 25624-38 (2014)
PMID: 25086049

Function and Biology Details

Reaction catalysed: 
An alpha-L-fucoside + H(2)O = L-fucose + an alcohol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
alpha-L-fucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 585 amino acids
Theoretical weight: 65.71 KDa
Source organism: Fusarium graminearum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: J9UN47 (Residues: 25-609; Coverage: 96%)
Gene name: FCO1
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand NAG 3 x NAG
Ligand TRS 6 x TRS
Ligand NA 3 x NA
Ligand GOL 4 x GOL
Ligand FUL 2 x FUL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 54.153Å b: 75.989Å c: 80.97Å
α: 105.69° β: 107.32° γ: 106.93°
R-values:
R R work R free
0.14 0.138 0.166
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Structure and function of microbial α-l-fucosidases: a mini review.
Wu et al. (2023)
16 other articles

4 mentions without citation

Structure and substrate specificity of a eukaryotic fucosidase from Fusarium graminearum.
Cao et al. (2014)
3 more