› 4eam

X-ray diffraction
1.7Å resolution

1.70A resolution structure of apo beta-glycosidase (W33G) from sulfolobus solfataricus

Released:
DOI: 10.2210/pdb4eam/pdb
PDB entry 4eam coloured by chain, front view.
PDB entry 4eam coloured by chain, side view.
PDB entry 4eam coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Designing allosteric control into enzymes by chemical rescue of structure.
Deckert K, Budiardjo SJ, Brunner LC, Lovell S, Karanicolas J
J Am Chem Soc 134 10055-60 (2012)
PMID: 22655749

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-149648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 56.63 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: P22498 (Residues: 1-489; Coverage: 100%)
Gene names: SSO3019, lacS
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand CL 6 x CL
Ligand MPD 6 x MPD
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P3121
Unit cell:
a: 167.7Å b: 167.7Å c: 96.398Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.168 0.186
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Allostery in Its Many Disguises: From Theory to Applications.
Wodak et al. (2019)
12 more

2 mentions without citation

Designing allosteric control into enzymes by chemical rescue of structure.
Deckert et al. (2012)
1 more