› 4al0

X-ray diffraction
1.16Å resolution

Crystal structure of Human PS-1

Released:
DOI: 10.2210/pdb4al0/pdb
PDB entry 4al0 coloured by chain, front view.
PDB entry 4al0 coloured by chain, side view.
PDB entry 4al0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of microsomal prostaglandin E2 synthase provides insight into diversity in the MAPEG superfamily.
Sjögren T, Nord J, Ek M, Johansson P, Liu G, Geschwindner S
Proc Natl Acad Sci U S A 110 3806-11 (2013)
PMID: 23431194

Function and Biology Details

Reactions catalysed: 
RX + glutathione = HX + R-S-glutathione
(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prostaglandin E synthase Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O14684 (Residues: 1-152; Coverage: 100%)
Gene names: MGST1L1, MPGES1, PGES, PIG12, PTGES
Sequence domains: MAPEG family
Structure domains: Membrane associated eicosanoid/glutathione metabolism-like domain

Ligands and Environments


Cofactor: Ligand GSH 1 x GSH
2 bound ligands:
Ligand PLM 3 x PLM
Ligand BOG 2 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: R3
Unit cell:
a: 76.742Å b: 76.742Å c: 123.401Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.122 0.122 0.13
Expression system: Spodoptera frugiperda

Quick links

Citations

10 review citations

Perspective of microsomal prostaglandin E2 synthase-1 as drug target in inflammation-related disorders.
Koeberle et al. (2015)
9 more

17 mentions without citation

Structural basis for catalysis at the membrane-water interface.
Dufrisne et al. (2017)
16 more