› 4lru

X-ray diffraction
1.6Å resolution

Crystal structure of glyoxalase III (Orf 19.251) from Candida albicans

Released:
DOI: 10.2210/pdb4lru/pdb
PDB entry 4lru coloured by chain, front view.
PDB entry 4lru coloured by chain, side view.
PDB entry 4lru coloured by chain, top view.
Source organism: Candida albicans SC5314
Primary publication:
A glutathione-independent glyoxalase of the DJ-1 superfamily plays an important role in managing metabolically generated methylglyoxal in Candida albicans.
Hasim S, Hussin NA, Alomar F, Bidasee KR, Nickerson KW, Wilson MA
J Biol Chem 289 1662-74 (2014)
PMID: 24302734

Function and Biology Details

Reaction catalysed: 
(R)-lactate = methylglyoxal + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyoxalase 3 Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 26.12 KDa
Source organism: Candida albicans SC5314
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5AF03 (Residues: 1-236; Coverage: 100%)
Gene names: CAALFM_C302610CA, CaO19.251, CaO19.7882, GLX3, orf19.251, orf19.7882
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P42212
Unit cell:
a: 92.23Å b: 92.23Å c: 59.367Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The cell biology of Parkinson's disease.
Panicker et al. (2021)
6 more

3 mentions without citation

A glutathione-independent glyoxalase of the DJ-1 superfamily plays an important role in managing metabolically generated methylglyoxal in Candida albicans.
Hasim et al. (2014)
2 more