Function and Biology Details
Reaction catalysed:
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150545 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
General negative regulator of transcription subunit 1
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: CCR4-NOT transcription complex subunit 1 CAF1-binding domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Length: 249 amino acids
Theoretical weight: 28.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P25655 (Residues: 755-1000; Coverage: 12%)
Sequence domains: CCR4-NOT transcription complex subunit 1 CAF1-binding domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Poly(A) ribonuclease POP2
Molecule details ›
Chain: B
Length: 286 amino acids
Theoretical weight: 33.15 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: CAF1 family ribonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H
Length: 286 amino acids
Theoretical weight: 33.15 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P39008 (Residues: 151-433; Coverage: 65%)
Sequence domains: CAF1 family ribonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SLS BEAMLINE X06DA
Spacegroup:
P212121
Expression system: Escherichia coli BL21(DE3)
