› 3p0w

X-ray diffraction
1.71Å resolution

Crystal structure of D-Glucarate dehydratase from Ralstonia solanacearum complexed with Mg and D-glucarate

Released:
DOI: 10.2210/pdb3p0w/pdb
PDB entry 3p0w coloured by chain, front view.
PDB entry 3p0w coloured by chain, side view.
PDB entry 3p0w coloured by chain, top view.
Source organism: Ralstonia pickettii 12J
Entry authors: Fedorov AA, Fedorov EV, Gerlt JA, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109732 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glucarate dehydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 470 amino acids
Theoretical weight: 51.18 KDa
Source organism: Ralstonia pickettii 12J
Expression system: Escherichia coli
UniProt:
  • Canonical: B2UIZ1 (Residues: 11-469; Coverage: 98%)
Gene name: Rpic_4449
Sequence domains: Enolase C-terminal domain-like
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand GKR 4 x GKR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 79.958Å b: 128.822Å c: 178.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.265
Expression system: Escherichia coli

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