› 3i48

X-ray diffraction
1.8Å resolution

Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound magnesium ions

Released:
DOI: 10.2210/pdb3i48/pdb
PDB entry 3i48 coloured by chain, front view.
PDB entry 3i48 coloured by chain, side view.
PDB entry 3i48 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus RN4220
Entry authors: Huseby M, Shi K, Kruse AC, Ohlendorf DH

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease/exonuclease/phosphatase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 36.09 KDa
Source organism: Staphylococcus aureus subsp. aureus RN4220
Expression system: Escherichia coli
UniProt:
  • Canonical: A7LAI8 (Residues: 35-330; Coverage: 95%)
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 62.9Å b: 68.577Å c: 126.559Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.22
Expression system: Escherichia coli

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