3g10

X-ray diffraction
2.6Å resolution

Structure of S. pombe Pop2p - Mg2+ and Mn2+ bound form

Released:

Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-130797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A) ribonuclease pop2 Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 37.32 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O74856 (Residues: 4-335; Coverage: 99%)
Gene names: SPCC18.06c, caf1, pop2
Sequence domains: CAF1 family ribonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P212121
Unit cell:
a: 53.124Å b: 53.991Å c: 95.646Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.23
Expression system: Escherichia coli