› 3f3g

X-ray diffraction
3.75Å resolution

Crystal structure of the nucleoporin pair Nup85-Seh1, space group P212121

Released:
DOI: 10.2210/pdb3f3g/pdb
PDB entry 3f3g coloured by chain, front view.
PDB entry 3f3g coloured by chain, side view.
PDB entry 3f3g coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A fence-like coat for the nuclear pore membrane.
Debler EW, Ma Y, Seo HS, Hsia KC, Noriega TR, Blobel G, Hoelz A
Mol Cell 32 815-26 (2008)
PMID: 19111661

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155502 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleoporin SEH1 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 351 amino acids
Theoretical weight: 39.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P53011 (Residues: 1-349; Coverage: 100%)
Gene names: SEH1, YGL100W
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Nucleoporin NUP85 Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 570 amino acids
Theoretical weight: 64.93 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P46673 (Residues: 1-570; Coverage: 77%)
Gene names: J1624, NUP85, RAT9, YJR042W
Sequence domains: Nup85 Nucleoporin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 105.377Å b: 106.487Å c: 358.636Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.272
Expression system: Escherichia coli

Quick links

Citations

31 review citations

The nuclear pore complex and nuclear transport.
Wente et al. (2010)
30 more