Online tutorial
Functional genomics II
Common technologies and data analysis methods
This online course introduces common technologies in functional genomics studies, including microarrays and next generation sequencing (NGS), with a special focus on RNA-sequencing (RNA-seq). We look at important considerations when designing your experiments, data analysis methods, and discuss when to use one technology over another.
Who is this course for?
This course is aimed at experimental or bench-based researchers working in the molecular life sciences who have little or no previous experience of functional genomics. An undergraduate knowledge of a subject related to the life sciences would be an advantage.
This is the second part of our functional genomics course. We recommend that you look at Functional genomics I: Introduction and designing experiments before starting this course. You may also be interested in Functional genomics III: Submitting your data and functional genomics databases.
What will I achieve?
By the end of the course you will be able to:
- Evaluate different functional genomics technologies and know when to use them
- List some of the considerations when designing your own functional genomics experiments using these technologies
- Describe the steps involved in data analysis of microarray and RNA-seq experiments
What resources do I need?
There are no specific resources required to complete this course.
DOI:
10.6019/TOL.FunGenII-c.2016.00001.1
Course contents
- Introduction
- Real-time PCR
- Microarrays
- What is Next Generation DNA Sequencing?
- RNA sequencing
- Biological interpretation of gene expression data
- Genotyping, epigenetic and DNA/RNA-protein interaction methods
- DNA/RNA-protein interactions
- Summary
- Quiz: Check your learning
- Your feedback
- Learn more
- References
How and when to access the course
All our courses are designed with flexibility in mind. You can access them for free at any time, just click on the "Enter Course" button.
It is up to you how you use the course; you can either study the full course or you can focus on sections that are relevant to you. To jump between sections, use the navigation bar on the left or the arrows at the bottom of the page. You can also choose whether to complete the course in one go, or over several visits.
The average time to read through the main body of the course is 3 hours (not including exercises and external links). The time may vary depending on your prior knowledge and how you choose to work through the course.
Sign up to track your learning and more
You can track your progress and keep a record of any completed learning by creating an account. This will give you a 'My learning' page, which will have three main tabs to help keep track of your progress:
Favourite: To add online tutorials to your Favourite tab, you can 'Mark as Favourite' on the front page of online tutorials, allowing you to return and complete them in the future.
In progress: The courses which you have started reading will be added to your 'In progress' tab. Use the 'Mark as complete' button at the end of the course pages to get started.Your ‘In progress’ tab gets updated as you progress through the course and will show you what percentage of the course you have finished and will let you resume the course from where you left on your last visit.
Completed: The courses which you have finished entirely will be added to the 'Completed' tab.
The results you gain from completing quizzes and other interactive content will also be added to your My learning page.
You can register anytime here or click a 'Favourite' or 'Mark as complete' button to start tracking your progress.
You can still access all the online tutorials and interactive content if you do not register, but you will not be able to track your progress.
Making the most of the course
Learning something new takes time and practice. We encourage you to:
- Use the activities and quizzes to help you check your learning, recall and apply key concepts. Look out for these icons
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- Revisit sections as and when you need them. Bookmark relevant pages in your browser or use the navigation panel to jump the relevant section.
Getting help and providing feedback
If something isn’t working or if you have a question get in touch by contacting us at trainonline@ebi.ac.uk
Tell us what you thought about the course (both good and bad!) using the “Feedback and help” button found at the top of each page.
Your feedback helps us ensure we are providing training that is relevant and useful for you.
For help and support on EMBL-EBI resources you can contact the helpdesk directly.
Learn more
You can explore other training on offer from EMBL-EBI on our website. We offer online courses, webinars, face-to-face courses and offsite training.