6vaq

X-ray diffraction
2.95Å resolution

Crystal structure of glucose-6-phosphate dehydrogenase V394L mutant in complex with catalytic NADP+

Released:

Function and Biology Details

Reaction catalysed:
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate 1-dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 515 amino acids
Theoretical weight: 59.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11413 (Residues: 1-515; Coverage: 100%)
Gene name: G6PD
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P41212
Unit cell:
a: 158.973Å b: 158.973Å c: 113.186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.222
Expression system: Escherichia coli BL21(DE3)